BIOCHEMISTRY ACS EXAM QUESTIONS WITH 100% CORRECT ANSWERS

BIOCHEMISTRY ACS EXAM QUESTIONS WITH 100% CORRECT ANSWERS Unfolds proteins and gives them uniform negative charge. - answerSDS Reacts with the N-terminus of the protein to produce a 2,4-dinitrophenol derivative that labels the first residue. Can repeat hydrolysis to determine sequential amino acids - answerFDNB Reduces disulfide bonds. - answerDTT Adds carboxymethyl group on free -SH groups. Blocks disulfide bonding. - answerIodoacetate Bonds between two -SH groups that form between 2° and 3° structure. - answerDisulfide- bonds Breaks disulfide bonds. - answerßME Formed from 2 α-helices twisted around each other. "Coiled coil". Cross-linked by disulfide bonds. - answerα-keratin Repeating sequence of Gly-X-Pro. 3 stranded "coiled coil". Contains gly core. - answerCollagen Less distinct areas of α and ß folding. - answerα/ß Protein Folding Two distinct areas of α and ß folding. - answerα+ß Protein Folding Electrons in Fe are "spread out" and result in larger atom. - answerHigh-Spin Fe Electrons are less "spread out" and are compacted by electron rich porphyrin ring. - answerLow-Spin Fe Heme is in high-spin state. H2O is bound to heme. - answerT-State Heme is in low-spin state. O2 is bound to heme. - answerR-State Greatly reduces hemoglobin's affinity for O2 by binding allosterically. - answerBPG Slope of Lineweaver-Burke Graph - answerKm/Vmax Y-intercept of Lineweaver-Burke Graph - answer1/Vmax X-intercept of Linewaever-Burke Graph - answer-1/Km Kcat - answerVmax/Et Slope of Competitive Inhibition - answerαKm/Vmax X-intercept of Competitive Inhibition - answer-1/αKm Y-intercept of Competitive Inhibition - answerDoesn't change Slope of Uncompetitive Inhibition - answerKm/Vmax Y-intercept of Uncompetitive Inhibition - answerα'/Vmax X-intercept of Uncompetitive Inhibition - answer-α'/Km Slope of Mixed Inhibition - answerαKm/Vmax Y-intercept of Mixed Inhibition - answerα'/Vmax X-intercept of Mixed Inhibition - answer-α'/αKm Saccharide in Starch - answerD-glucose Has α-1,4-linkages - answerStarch Has α-1,4-linkages that form a coiled coil - answerAmylose Has α-1,4-linkages with periodic α-1,6-linkages ever