BIOL 111 Lab Exam 1 2024 with 100% correct answers

What is a blank? - correct answer Has everything except what you are measuring It acts as a zero value and an accurate difference. What does Bradford reagent bind to? What colors does it change when bound or unbound? How can you visually tell the amount of proteins in a test tube? - correct answer It is usually brown in color, but when it binds to proteins it turns blue. The deeper/darker the blue color is, the more proteins there are. Why is R2 important? What is a good R2 value? - correct answer The value shows how good your linear regression line is. The closer the value is to 1, the lower the standard of error. A good value is typically between 0.98-1.00 Why do you remove the last two points on the standard curve? - correct answer There is a point of saturation, where the graph plateaus. Including the last two points on the plateau of the graph lowers the R^2 value, therefore removing the two points makes the data have a more predictable correlation and increases the R^2 value. Why do we use a standard curve? - correct answer To determine the concentration of protein in an unknown sample by comparing it to known values. It provides the absorbance values for a range of known protein concentrations. Stock solution vs. Working solution - correct answer Stock solution is the original concentration Working solution is the dilution of the stock