HISTOTECHNOLOGY(fully solved)graded A+ 2023/2024

HISTOTECHNOLOGY Art and science performed to produce a tissue section of good quality that will enable pathologist to diagnose the presence or absence of a disease. Histotechnology Used to view slides not seen by the naked eye microscope Used by pathologist to view slides to identify a disease or process. microscope Used by histotechnologist to view slides FOR QUALITY CONTROL microscope Basic instrument used in microtomy (the process by which processed tissue, most commonly a paraffin embedded tissue, is trimmed and cut into uniformly thin slices or "sections" to facilitate studies under a microscope Microtome The first and most important step in operation of any piece of equipment is to read the manual that accompanies the equipment. instrumentation file of equipment in the lab contains? a. Name, manufacturer, model number, and serial number b. Record of preventive maintenance performed, as prescribed by the manufacturer. C. Record of service calls and repairs performed D. copy of operating manual three essential parts of microtomes 1. block holder 2. knife/ knife carrier 3. Pawl, ratchet feed, Whee; and Adjustment screws microtome part that hold the tissue in position block holder essential part of microtome for actual cutting of tissue secretions knife/ knife holder essential part of microtome to line up the tissue block in proper position with knife, adjusting thickness of tissue of successive sections Pawl, ratchet feed, Whee; and Adjustment screws Types of Microtomes rocking rotary sliding freezing ultrathin type of microtome used for cutting serial secretions of large block paraffin embedded tissue rocking (cambridge) Simplest among the different type of microtome rocking (cambridge) type of microtome for cutting paraffin embedded sections rotary (minot) Most common type of microtome used for both routine and research laboratories rotary (minot) type of microtome for cutting celloidin embedded sections sliding Most dangerous type of microtome due to movable exposed knife. sliding Two types of Sliding Microtome Base Sledge Standard Sliding