11D: Explore basic DNA techniques and the use of genetic engineering technologies.
Basic DNA techniques and genetic engineering technology
Introduction
In this assignment what is discussed is the gel electrophoresis to separate the amplified
DNA practical and how the procedure went and what was done and the steps need to be
followed and completed. What is also discussed is genetic engineering technology, the
benefits of it, how it is also used in the industry and in medicine, what is covered about this
topic is the future uses of this technology and its strengths and weaknesses and how it is
used in our current modern day society.
Gel electrophoresis to separate the amplified DNA Practical
Equipment list:
Melted agarose
Comb with teeth
Micropittetes
Buffer solution
Gel tray
Rubber gaskets
Method:
The rubber gaskets were attached to the gel tray and the comb teeth was placed in secure
way that when the melted agarose was poured it wouldn't move
The melted agarose was poured into the gel tray and was left to set for 10 minutes while the
gel was setting. We prepared the mircopittetes for use.
After 10 minutes the agarose was set and the comb and rubber gaskets were removed from
the gel tray.
Then we began to load the well with 20μl of each dye for each tip used; it was discarded so
that it was not reused for the other dye.
When we completed loading the dye in the wells, the gel trays were then placed in an
electrophoresis unit where 150ml of solution was approximately added in the box to cover
the gel surface. The lid was then placed on top.
Any spillages were then cleaned up and the terminals were then connected carefully. The
power supply was then plugged in and the 75V was selected and turned on.
We observed the box and let the power run through the buffer solution for 30 minutes. When
the dyes moved across the gel about 1cm the power supply was then turned off and
unplugged and was disconnected from the electrophoresis box.
, Observation:
Basic DNA techniques and genetic engineering technology
Introduction
In this assignment what is discussed is the gel electrophoresis to separate the amplified
DNA practical and how the procedure went and what was done and the steps need to be
followed and completed. What is also discussed is genetic engineering technology, the
benefits of it, how it is also used in the industry and in medicine, what is covered about this
topic is the future uses of this technology and its strengths and weaknesses and how it is
used in our current modern day society.
Gel electrophoresis to separate the amplified DNA Practical
Equipment list:
Melted agarose
Comb with teeth
Micropittetes
Buffer solution
Gel tray
Rubber gaskets
Method:
The rubber gaskets were attached to the gel tray and the comb teeth was placed in secure
way that when the melted agarose was poured it wouldn't move
The melted agarose was poured into the gel tray and was left to set for 10 minutes while the
gel was setting. We prepared the mircopittetes for use.
After 10 minutes the agarose was set and the comb and rubber gaskets were removed from
the gel tray.
Then we began to load the well with 20μl of each dye for each tip used; it was discarded so
that it was not reused for the other dye.
When we completed loading the dye in the wells, the gel trays were then placed in an
electrophoresis unit where 150ml of solution was approximately added in the box to cover
the gel surface. The lid was then placed on top.
Any spillages were then cleaned up and the terminals were then connected carefully. The
power supply was then plugged in and the 75V was selected and turned on.
We observed the box and let the power run through the buffer solution for 30 minutes. When
the dyes moved across the gel about 1cm the power supply was then turned off and
unplugged and was disconnected from the electrophoresis box.
, Observation:
