Lab 2 Culturing & Aseptic Technique. BIO250L

Lab 2 Culturing & Aseptic Technique. BIO250L Student Name: Access Code (located on the underside of the lid of your lab kit): AC-CJFUVAG Pre-Lab Questions 1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one experimental way you can test your practices to confirm that you are using proper aseptic technique? By burning the loop or using agar slants, aseptic procedures can be evaluated for applicability. The loop is flamed until it gets red-hot, then cooled before taking up the organisms to be transferred from the bacterial culture. So that no bacterial aerosol is formed, the hot loop is left in the tube for a few seconds before extracting the inoculum. 2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment? a. When sterilizing culture material in an autoclave, the wet heat approach is favored. In an autoclave, the material is heated using pressure steam, which is an excellent method for destroying germs, spores, and viruses at 100°C.. b. Dry heat approach kills bacteria by oxidizing cells, it uses more energy and is carried out at a higher temperature of 121°C for effective sterilization. c. Filtration, which involves passing a solution through a filter with a pore diameter of 0.2 m, is another way of sterilizing liquids without heating. Heat-fused glass funnels or cellulose membranes can be used as sterilization filters. Viruses can flow through these filters, so filtration isn't the best way of sterilizing. Student Name: Access Code (located on the underside of the lid of your lab kit): AC-CJFUVAG Pre-Lab Questions 1. Proper aseptic technique is crucial to ensuring growth of pure bacterial colonies. What is one experimental way you can test your practices to confirm that you are using proper aseptic technique? By burning the loop or using agar slants, aseptic procedures can be evaluated for applicability. The loop is flamed until it gets red-hot, then cooled before taking up the organisms to be transferred from the bacterial culture. So that no bacterial aerosol is formed, the hot loop is left in the tube for a few seconds before extracting the inoculum. 2. In a laboratory setting, what are three ways you can properly sterilize culturing equipment? a. When sterilizing culture material in an autoclave, the wet heat approach is favored. In an autoclave, the material is heated using pressure steam, which is an excellent method for destroying germs, spores, and viruses at 100°C.. b. Dry heat approach kills bacteria by oxidizing cells, it uses more energy and is carried out at a higher temperature of 121°C for effective sterilization. c. Filtration, which involves passing a solution through a filter with a pore diameter of 0.2 m, is another way of sterilizing liquids without heating. Heat-fused glass funnels or cellulose membranes can be used as sterilization filters. Viruses can flow through these filters, so filtration isn't the best way of sterilizing.