BACE Exam practice: Multiple Choice 2023

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - (50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - 1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide lanA PCR product and the plasmid with appropriate restriction enzymes 3) use ligase to "paste" LanA into a plasmid 4) transform E. coli with plasmid containing LanA how much tetracycline is needed to prepare a 100 mL of 100 mg /ml tetracycline (w/v)? - (100mL)(100mg/ML) = 10000mg (10g) after performing the PCR reaction with the primers she designed to amplify lanA, the same technician found her PCR reaction contained two distinct bands, one at the expected size of 1200 kilobase pairs, and another unexpected band at 2000 Kilobase pairs. what is the most likely reason there's an unexpected bands and her PCR reaction? - she used an annealing temperature that was too low in a biotechnology lab, tap water is best to use for: - first rinse when washing glassware the order of amino acids on a polypeptide chain is encoded by: - mRNA how do DNA molecules vary from one species to another? - the number of genes and non-coding regions what is agar? - solid media on which bacteria grows what part of an amino acid differs from one to the other? - R group how are the proteins in fractions collected during column chromatography visualized? - using polyacrylamide gel electrophoresis (SDS PAGE) which of the following is an mRNA codon? - UAU what is the purpose of SDS when used in SDS page electrophoresis? - denature the proteins and give them a negative charge which department is in charge of testing biopharmaceutical products to ensure its safety and potency? - quality control (QC) lipids provide which of the following functions for cells? - stored energy which of the following is a monosaccharide commonly used as an energy source for cells in cell culture media? - glucose where does translation occur in prokaryotes? - at the ribosomes in aerobic respiration carbohydrates are ultimately broken down into: - CO2, H2O, ATP most ATP in eukaryotic cells is produced in the: - mitochondria an aqueous solution and yields a hydrogen ion when ionized - acid defined as "the moles of solute per liter of solution" - molarity a solution that resists changes in PH - buffer