Khansa Zahid
Id number: 40176861
Unit 11b
, P2/m2
Garlic root tip procedure:
Safety procedures:
1. Wear goggles
2. Acetic orcein stain is corrosive so handle with care
Method :
1.Add One mol dm3 of hydrochloric acid in a tiny container should be placed in a
temperature regulated water bath that is heated to 55 °C. 15 minutes should pass for the
acid to heat up to the water bath's level in the container.
2. Insert a garlic clove to ensure that the root are immersed in the 55 °C hydrochloric acid
just at the peak of the container. Give the roots five minutes in the acid.
3. Pull out all the clove and vigorously wash the roots with tap water once the five minutes
have passed. Snip off numerous root tips that are between 5 and 10 mm long using a set of
scissors. Allow particles to drop into a little acetic acid container ontop of a white tile.
Ensure the root tips are completely submerged in the dye by using the scissors. Cover the
container with a cover or a piece of lab elastic fabric. To avoid fluid from ejecting while
heated, caps ought to have a needle prick hole or, if they're screw caps, must be just a little
bit slack.
4. Further increase the coloring, immerse the vial holding the root tips in acetic acid in the 55
°C water bath for five minutes.
5. Following waiting for 5 minutes, remove the tips from the vial with tweezers and put those
on a glass slide. Place a small amount of water on the slide's root tip. To slightly stretch out
the cells, macerate (tear apart) the root tip. Use a coverslip to conceal. Put the bottle of
stain's cover back on and give it to the professor as directed.
6. Crush the tissue by lighlty pressing on the sheet while wrapping the slides across several
layers of paper towels. To avoid breaking the coverslip, be careful never to bend the slide
when you apply pressure.
Id number: 40176861
Unit 11b
, P2/m2
Garlic root tip procedure:
Safety procedures:
1. Wear goggles
2. Acetic orcein stain is corrosive so handle with care
Method :
1.Add One mol dm3 of hydrochloric acid in a tiny container should be placed in a
temperature regulated water bath that is heated to 55 °C. 15 minutes should pass for the
acid to heat up to the water bath's level in the container.
2. Insert a garlic clove to ensure that the root are immersed in the 55 °C hydrochloric acid
just at the peak of the container. Give the roots five minutes in the acid.
3. Pull out all the clove and vigorously wash the roots with tap water once the five minutes
have passed. Snip off numerous root tips that are between 5 and 10 mm long using a set of
scissors. Allow particles to drop into a little acetic acid container ontop of a white tile.
Ensure the root tips are completely submerged in the dye by using the scissors. Cover the
container with a cover or a piece of lab elastic fabric. To avoid fluid from ejecting while
heated, caps ought to have a needle prick hole or, if they're screw caps, must be just a little
bit slack.
4. Further increase the coloring, immerse the vial holding the root tips in acetic acid in the 55
°C water bath for five minutes.
5. Following waiting for 5 minutes, remove the tips from the vial with tweezers and put those
on a glass slide. Place a small amount of water on the slide's root tip. To slightly stretch out
the cells, macerate (tear apart) the root tip. Use a coverslip to conceal. Put the bottle of
stain's cover back on and give it to the professor as directed.
6. Crush the tissue by lighlty pressing on the sheet while wrapping the slides across several
layers of paper towels. To avoid breaking the coverslip, be careful never to bend the slide
when you apply pressure.
