BIOS 242 Week 4 Lab 2; Fomite Transmission

Lab8:FomiteTransmission LearningObjectives: • Definefomiteandhowbacteriaaretransferredviafomites. • Explaintheroleoffomitetransmissioninthehospital. • Definenosocomialinfections,HAIs(hospitalacquiredinfections),andIatrogenicinfections. Introduction: Materials:Agarplate,Marker,ruler,sterileswabs Method: 1. Obtainonenutrientagaranddividethebackoftheplateinto8sectionsusingrulerandmarker.Labeleachsection 1-8. 2. Usinganewsterileswab,swab8differentinanimateobjectsfoundinthelabareaandstreaktheagar plates. 3. Recordthenameoftheswabbedobjectsinthelabreport. 4. Incubatetheagarplatesat37◦Cfor24-48hours. 5. Sealtheplateswithparafilmanddonotopentheplates. 6. Makeobservationsusingsealedplatesonly. 7. Uselabsafetyprotocolatalltimeswhileobservingeachsectionsforgrowthonthescaleof0-5.Ifthereisnogrowth,reportitaszero.Reportexcessivegrowthas5. 8. Onthelabreport,describeobservationsregardingappearance,color,shapeandsizeofthecolonies. 9. Onceobservationiscompleted,immediatelyputtheplatesinappropriatewastecontainer(biohazardbag)forautoclaving. LabReport: Purpose:Toseethebacteria,viruses,orfungigrowthoninanimateobjectswhichwouldrefertoasfomite. Results: GrowthScale:0=NoGrowth;5=MaximumGrowth Questions: 1. Differentiatebetweennosocomial,HAI,andiatrogenicinfections. 2. Describethreewaysanursecouldtransfermicroorganismstotheirpatients. 3. Differentiatebetweencontact,vehicle,andvectortransmission. 4. Wasthereequalamountofgrowthonallsections?Wouldallmicroorganismsonafomitebeabletogrowon nutrientagar?Whyorwhy not?