BIOS 242 Week 3 Discussion; Culturing Microorganisms - Amoeba

Week 3 Discussion: Culturing Microorganisms Required Resources Read/review the following resources for this activity: • Textbook: Chapters 7, 8 • Weekly Concepts Initial Post Instructions Class, let’s discuss the categories that organisms can be grouped in based on their nutritional requirements. Find one microorganism, either a prokaryote or eukaryote, and describe the environment in which it lives. (Does it live underwater? On skin? In soil? Give as many details as possible!) To complete your initial post, you will then use the vocabulary we discussed to classify it based on its nutritional needs and environmental requirements. (Is it a halophile? A chemoheterotroph? Use as many terms as you can!) Follow-Up Post Instructions For your second post, reply to your peer’s post to propose the best way to culture their microorganisms with explanation. Explain how the conditions and culture media you choose will meet the nutritional and environmental needs of the microorganism. (Is there a particular temperature you should use? Is CO2needed? What medium would be best?) Respond to at least one peer or the instructor. Further the dialogue by providing more information and clarification. Writing Requirements • Minimum of 2 posts (1 initial & 1 follow-up) • Minimum of 2 sources cited (assigned readings/online lessons and an outside source) • APA format for in-text citations and list of references HelloClassandProfessor, Thisweekdiscussion isbasedonCulturingMicroorganisms Amoeba Amoeba are eukaryotic microorganisms. They live in soil, waters and in trees butonebarely noticesthem as they are notvisible onnaked eyes dueto theirmicroscopicnature (Horn & Wagner, 2017). Amoeba can also be found in humans especially thosewhodrink contaminatedwater containingthem.Entamoebahistolyticaisa speciesofAmoeba that find its way in the human intestines. Due to Amoeba diverse habitats thisinfluences also what it consumes. The Amoeba that are found in aquatic environmentmostly consumes algae as algae are mostly found under water (Marjorie & Heidi, 2020).Amoebaalso consume bacteriainlargequantities,especiallythose found on soilparticles. Amoebahave alsospecialmechanismsthatdetects thesurroundingenvironment when there are harsh environmental conditions Amoeba undergoesencystment.EncystmentisachangewheretheAmoebachangesitsshapeandassumesacircularshape,itdrainsoutexcesswaterandsecretesacyst tissue(membrane)thatprotectsitfromtheextremeenvironment(Horn&Wagner,2017).Once the environment becomes conducive for the Amoeba, the membrane raptures andtheAmoebareturnsbacktoitsoriginalshape. Amoeba’s modeofnutrition isholozoic nutrition.Ittakes in(ingest),digests and then egests the food material to the host. However, Amoeba lacks aspecialized organ for nutrition but the whole nutrition process is carried out on its bodysurface mostly by the pseudopods (Marjorie & Heidi, 2020). The pseudopods are alsovital in the movement of Amoeba as they coils to facilitate its movement. Amoeba getsnourishedthrough phagocytosiswhich is themode ofeatingfoodby swallowing theentireorganismtheydecidetofeedon. Whatisthenutritionalmodeoffungi? Fungi have heterotrophic mode of nutrition. Fungi lacks chlorophyll and thereforetheycannotmanufacturetheirownfoodbutdependsonlivingordeadorganicmatterfortheirsource ofenergy (Marjorie&Heidi, 2020).Fungiare classifiedinto foursubgroupsdepending ontheirnutrition mode. Saprophytes arefungithat feed on deadorganic matter.Theyarefurther,classifiedintotwosubgroups,thosethatdeveloponthe outer surface oforganicmatter are called the ectophyticsaprophytes whilethosethat develop inside the matter are endophytic saprophytes. Parasitic fungi depends on ahostfornutrients.Symbiotic fungi havea mutual relationship with other organisms. Predaciousfungifeedonlive organisms suchas thenematodes. Part2 UsingmyPathogenfromlasttwoweeks. Lassa mammarenavirus is a virus that causes Lassa hemorrhagic fever. TheprimaryhostforLassamammarenavirus isMastomysrodents.Theviruscan surviveoutsidethehostas anaerosol inan environmentwithlow relativehumidity(30% RH)(VelocityEHS,2017).ThenutritionalmodeforLassamammarenavirus canbedescribedasparasitic.Thisisbecausethevirushighlydependsonnutritionalsubstrates from its host. A creature that lives on or inside its host's body is referred to asaparasitesinceitfeedsoffofthehost. Theparasitethatacquiresthefoodisknownasa parasite,andthecreature fromwhose bodythe foodistakenisknownas thehostparasite (Marjorie & Heidi, 2020). Lassa mammarenavirus usually targets macrophages,Antigen-presenting cellsand dendriccellsofthehost.Theinfection ofthiscells isinfluencedby the factthattheyare widely distributed in themucosal tissues andskinwhichallowthefirstreplicativecycles. References; Horn,M.,&Wagner,M.(2017).Bacterialendosymbiontsoffree‐livingamoebae1. JournalofEukaryoticMicrobiology,51(5),509-514. Marjorie, K., C. & Heidi, S., 2020. Microbiology: A Systems Approach 6th Edition.Publisher: McGraw-HillEducation. VelocityEHS,2017.Lassa Virus.PathogenSafetyDataSheet-InfectiousSubstances.Available at: