BIOD 171 MODULE 3 EXAM LATEST 2021/2022.

BIOD 171 MODULE 3 EXAM LATEST 2021/2022. 1. True or False: A nanometer (10-9) is longer than a micrometer (10-6). FALSE: A nanometer is 1,000 times smaller than a micrometer. 2. Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low resolution means two (or more) objects may appear as one. Contrast is the difference in light absorbance between two objects. Poor contrast gives a high background and makes the visualization of multiple objects difficult. For instance, trying to identify 2 dark colored objects at night (low light = low contrast) versus the same 2 objects in the middle of a sunny afternoon (bright light against 2 dark objects = high contrast). 3. Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the magnification of a sample. OBJECTIVE: Only the Oculars (eye piece) and the objectives contribute to the magnification of the sample. Since the eyepiece is fixed, only the objectives could be altered. 4. What is the total magnification (relative to your eye) of a sample imaged with with a 20 objective and A 15X eye piece? Show your math. 20 x 15 = 300x magnification QUESTION 5 FALSE: QUESTION 6 Which of the following could be seen clearly by the unaided eye? Select all that apply. The unaided eye can, on average, clearly resolve objects > 100 μm QUESTION 7 Resolution and contrast are two critical factors that influence your ability to see an object. Explain each. Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance the magnification of a sample. Only the oculars (eyepiece) and the objectives contribute to the magnification of the sample. Since the eyepiece is fixed, only the objectives could be altered. What is the total magnification (relative to your eye) of a sample imaged with a 20x objective and a 15x eyepiece? Show your math. True or False: A cell that is adherent, flat (thin), and unstained is easily identified using bright field microscopy.