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BIOS 242 Week 2 iLab: Aseptic Technique
ASEPTIC TECHNIQUE
Introduction
The purpose of this lab is to culture cells successfully without contamination using
aseptic technique. If contamination occurs, the lab experiment will be inaccurate.
Biological contamination can happen via dirty supplies, airborne toxins, and unkempt
workstations. This aseptic technique lab is designed to guide you through a successful
(uncontaminated) lab experiment. With these aseptic techniques it will help to yield a
level of protection for the cell culture. Aseptic techniques require sterile media, sterile
agents, proper hygiene, and a clean workspace.
Findings: Mannitol Fermentation test 100% positive.
Procedure
Conduct Contamination Test:
1. Click the New Unknown button. A window will open asking you to enter a
label and select a subgroup.
2. Type Technique1 in the “Enter a Label” line.
3. From the Subgroup dropdown menu, select Asceptic Technique. DO NOT
CLICK Auto-inoculation allowed. Click OK.
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4. Record details of your case study scenario.
5. Record the Gram Reaction by clicking the appropriate radio button. Click
Record Gram Reaction.
6. You will need to indicate the results of the Gram Stain in your Lab Report.
7. From the Media dropdown menu, select Phenol Red Glucose Broth Durham
Tube. The Media dropdown menu is to the far right of the New Unknown
button.
8. Enter PhenolRedGlucose in the Medium Label window to label your sample.
Click OK. Two tubes will appear on your workbench. Take a careful look at
the sample tube (the one on the right.) Is there media in the Durham Tube
(this is the tube that is upside down in the medium.)
9. Note the status of the Traffic Lights for Inoculation and Contamination in the
upper right hand corner of the lab software window.
10. Right click on the phenol red tube (the tube on the right) and select Remove
caps/lids.
iLab: Aseptic Technique and Bacterial Anatomy and Morphology Page 4
11. Look at the Inoculation/Contamination traffic lights. What color
are these lights?
12. Right click on the phenol red tube and select Replace caps/lids. Look
at the Inoculation/Contamination traffic lights. How has the color
of each light changed? Record these changes for your lab report.
13. Using your cursor, drag the phenol red tube and place it on the 37
degree incubator (this is the incubator on the right side of the
BIOS 242 Week 2 iLab: Aseptic Technique
ASEPTIC TECHNIQUE
Introduction
The purpose of this lab is to culture cells successfully without contamination using
aseptic technique. If contamination occurs, the lab experiment will be inaccurate.
Biological contamination can happen via dirty supplies, airborne toxins, and unkempt
workstations. This aseptic technique lab is designed to guide you through a successful
(uncontaminated) lab experiment. With these aseptic techniques it will help to yield a
level of protection for the cell culture. Aseptic techniques require sterile media, sterile
agents, proper hygiene, and a clean workspace.
Findings: Mannitol Fermentation test 100% positive.
Procedure
Conduct Contamination Test:
1. Click the New Unknown button. A window will open asking you to enter a
label and select a subgroup.
2. Type Technique1 in the “Enter a Label” line.
3. From the Subgroup dropdown menu, select Asceptic Technique. DO NOT
CLICK Auto-inoculation allowed. Click OK.
, 2
4. Record details of your case study scenario.
5. Record the Gram Reaction by clicking the appropriate radio button. Click
Record Gram Reaction.
6. You will need to indicate the results of the Gram Stain in your Lab Report.
7. From the Media dropdown menu, select Phenol Red Glucose Broth Durham
Tube. The Media dropdown menu is to the far right of the New Unknown
button.
8. Enter PhenolRedGlucose in the Medium Label window to label your sample.
Click OK. Two tubes will appear on your workbench. Take a careful look at
the sample tube (the one on the right.) Is there media in the Durham Tube
(this is the tube that is upside down in the medium.)
9. Note the status of the Traffic Lights for Inoculation and Contamination in the
upper right hand corner of the lab software window.
10. Right click on the phenol red tube (the tube on the right) and select Remove
caps/lids.
iLab: Aseptic Technique and Bacterial Anatomy and Morphology Page 4
11. Look at the Inoculation/Contamination traffic lights. What color
are these lights?
12. Right click on the phenol red tube and select Replace caps/lids. Look
at the Inoculation/Contamination traffic lights. How has the color
of each light changed? Record these changes for your lab report.
13. Using your cursor, drag the phenol red tube and place it on the 37
degree incubator (this is the incubator on the right side of the
