Summary BIO 171_Lab_4_Notebook PL #4 Introduction Growth Media

Lab 4 Notebook
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Title: PL #4 Introduction Growth Media
Objective: T o learn and understand different types of growth media in order to isolate and identify unknown samples
Procedure: 4- Phase Dilution Streaking: Clonal Isolation
1.Using sterile loop spread culture in area #1 ONCE
2.Using a NEW sterile loop drag through the end of area #1 ONCE
3.Using a NEW sterile loop drag through the end of area #2 ONCE
4.Using a NEW sterile loop drag through the end of area #3 ONCE
*Use a back and forth pattern to dilute the culture in each zone.
*Invert plate and incubate overnight at 37C
Non - selective agar
Quadrant Growth: Rapid test for multiple isolates
1.Using sterile loop spread unknown culture A in area #1
2.Using a new sterile loop spread unknown culture B in area #2
3.Using a NEW sterile loop spread unknown culture C in area #3
4.Using a NEW sterile loop spread unknown culture D in area #4
*Use a back and forth pattern to dilute the culture in each zone.
* Invert plate and incubate overnight at 37C
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