ACS Biochemistry First Semester Exam | Actual Questions and Answers
Latest Updated 2024/2025 (Graded A+)
Buffer Order and Henderson Hasselbach - ✔✔Water, Buffer, Salt, Base
pH = pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
Ramachandran plots - ✔✔Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance
Protein structure of a helix, B sheet, reverse turn - ✔✔a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - ✔✔3.6
Super secondary structure - ✔✔helix turn helix
parallel vs. antiparallel - ✔✔parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - ✔✔pH at which a particular molecule carries no net electrical charge.
Protein Separation Techniques (ALL) - ✔✔Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
, Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG
Protein Separation Techniques (H) - ✔✔Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - ✔✔Salting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - ✔✔Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) - ✔✔ion exchange
separation based on charge; + proteins stick to - beads
Protein Separation Techniques (SG) - ✔✔Size Exclusion/Gel Filtration
separation based on size
Cuts (protein separation techniques) - ✔✔cut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)
Dialysis - ✔✔protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Electrophoresis - ✔✔gels - move by size with electric current
1. Isoelectric focusing - purely pH
Latest Updated 2024/2025 (Graded A+)
Buffer Order and Henderson Hasselbach - ✔✔Water, Buffer, Salt, Base
pH = pKa + log(A/HA)
pKa decrease as temp increase so pH decrease
Ramachandran plots - ✔✔Φ = N-R
ψ = C-R
optimal angle to prevent steric hinderance
Protein structure of a helix, B sheet, reverse turn - ✔✔a: LEMARKHQ
B: VICYFTW
RT: PSDNG
how many amino acids in alpha turn helix - ✔✔3.6
Super secondary structure - ✔✔helix turn helix
parallel vs. antiparallel - ✔✔parallel wedges face same way
antiparallel face opposite
Isoelectric point (pI) - ✔✔pH at which a particular molecule carries no net electrical charge.
Protein Separation Techniques (ALL) - ✔✔Homogenization
Salting Out
Affinity Chromatgraphy
Ion Exchange
, Size Exclusion/Gel Filtration
H, S, A, I, SG
He said all individuals SinG
Protein Separation Techniques (H) - ✔✔Homogenization
disrupt cells, sonicator, centrifuge
Protein Separation Techniques (S) - ✔✔Salting out
different proteins precipitate out at different salt concentrations
Protein Separation Techniques (A) - ✔✔Affinity chromatography
add a tag; histidine binds; see how well there is an affinity for the group
Protein Separation Techniques (I) - ✔✔ion exchange
separation based on charge; + proteins stick to - beads
Protein Separation Techniques (SG) - ✔✔Size Exclusion/Gel Filtration
separation based on size
Cuts (protein separation techniques) - ✔✔cut out what dont need/isnt protein
(use 40% concen. then keep liquid; if use 60% concen. then keep pellet)
Dialysis - ✔✔protein separation based on bad with pores
1. Moles in bag + moles in buffer
2. Divide moles by total volume - new concentration
Electrophoresis - ✔✔gels - move by size with electric current
1. Isoelectric focusing - purely pH
