MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
The process of importing free DNA into cells is known as __________.
a. tranposition
b. conjugation
c. specialized transduction
d. generalized transduction
e. transformation - (ANSWER)transformation
Which of the following bacteria requires artificial assistance to become competent to take up DNA?
a. Bacillus
b. E. coli
c. Haemophilus
d. Neisseria
e. Streptococcus - (ANSWER)E. coli
A special sigma factor, __________, is used to transcribe genes encoding the translocasome necessary
for cell competency.
a. SigH
b. Sig70
c. SigT
d. SigC
e. Sig20 - (ANSWER)SigH
__________ plasmids usually contain an oriT-like DNA replication origin that is recognized by the
conjugation apparatus of the transferable plasmid.
a. Mobilizable
b. Fertility
c. Hfr
,MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
d. F'
e. Conjugative - (ANSWER)Mobilizable
During conjugation the DNA passes through the __________, NOT the __________.
a. membranes; pilus
b. pilus; membranes
c. pilus; phage
d. phage; pilus
e. F factor; phage - (ANSWER)membranes; pilus
The transfer of F factor in E. coli from one donor cell to a __________ cell results in two __________
cells.
a. donor; donor
b. recipient; recipient
c. donor; recipient
d. recipient; donor
e. recipient; Hfr - (ANSWER)recipient; donor
F factor contains how many origins of replication?
a. one
b. two
c. three
d. four
e. none - (ANSWER)two
The 5'-end of the nicked strand of F factor moves through a pore in the conjugation complex by a
process driven by __________, which synthesizes a replacement strand in the donor cell using the intact
strand as template.
,MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
a. DNA pol I
b. DNA pol II
c. DNA pol III
d. ligase
e. Hfr - (ANSWER)DNA pol III
Because the F plasmid can exist in extrachromosomal and integrated forms, it is sometimes called a/an
__________.
a. episome
b. F'
c. Hfr
d. lysogen
e. tranducing phage - (ANSWER)episome
Approximately how long does it take for an Hfr strain to transfer its entire chromosome?
a. 30 minutes
b. 60 minutes
c. 100 minutes
d. 180 minutes
e. 300 minutes - (ANSWER)100 minutes
The recipient of Hfr conjugation __________ becomes an F+ or Hfr cell.
a. rarely
b. usually
c. always
d. never
e. frequently - (ANSWER)rarely
, MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
Interrupted mating involves breaking __________ bridges using a blender.
a. transformation
b. transduction
c. conjugation
d. transposition
e. bacteriophage - (ANSWER)conjugation
A derivative F plasmid that contains host DNA is called an:
a. F plasmid
b. F+ plasmid
c. F- plasmid
d. F' plasmid
e. Hfr plasmid - (ANSWER)F' plasmid
Some conjugation systems use __________ to stimulate transfer of genetic information.
a. transformation
b. pheromones
c. transduction
d. transposition
e. reversion - (ANSWER)pheromones
Salmonella can conduct interspecies __________ with E. coli.
a. transduction
b. transformation
c. transposition
LATEST UPDATE ALREADY GRADED A+
The process of importing free DNA into cells is known as __________.
a. tranposition
b. conjugation
c. specialized transduction
d. generalized transduction
e. transformation - (ANSWER)transformation
Which of the following bacteria requires artificial assistance to become competent to take up DNA?
a. Bacillus
b. E. coli
c. Haemophilus
d. Neisseria
e. Streptococcus - (ANSWER)E. coli
A special sigma factor, __________, is used to transcribe genes encoding the translocasome necessary
for cell competency.
a. SigH
b. Sig70
c. SigT
d. SigC
e. Sig20 - (ANSWER)SigH
__________ plasmids usually contain an oriT-like DNA replication origin that is recognized by the
conjugation apparatus of the transferable plasmid.
a. Mobilizable
b. Fertility
c. Hfr
,MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
d. F'
e. Conjugative - (ANSWER)Mobilizable
During conjugation the DNA passes through the __________, NOT the __________.
a. membranes; pilus
b. pilus; membranes
c. pilus; phage
d. phage; pilus
e. F factor; phage - (ANSWER)membranes; pilus
The transfer of F factor in E. coli from one donor cell to a __________ cell results in two __________
cells.
a. donor; donor
b. recipient; recipient
c. donor; recipient
d. recipient; donor
e. recipient; Hfr - (ANSWER)recipient; donor
F factor contains how many origins of replication?
a. one
b. two
c. three
d. four
e. none - (ANSWER)two
The 5'-end of the nicked strand of F factor moves through a pore in the conjugation complex by a
process driven by __________, which synthesizes a replacement strand in the donor cell using the intact
strand as template.
,MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
a. DNA pol I
b. DNA pol II
c. DNA pol III
d. ligase
e. Hfr - (ANSWER)DNA pol III
Because the F plasmid can exist in extrachromosomal and integrated forms, it is sometimes called a/an
__________.
a. episome
b. F'
c. Hfr
d. lysogen
e. tranducing phage - (ANSWER)episome
Approximately how long does it take for an Hfr strain to transfer its entire chromosome?
a. 30 minutes
b. 60 minutes
c. 100 minutes
d. 180 minutes
e. 300 minutes - (ANSWER)100 minutes
The recipient of Hfr conjugation __________ becomes an F+ or Hfr cell.
a. rarely
b. usually
c. always
d. never
e. frequently - (ANSWER)rarely
, MCB 3020 MICROBIOLOGY EXAM 4 QUESTIONS AND ANSWERS
LATEST UPDATE ALREADY GRADED A+
Interrupted mating involves breaking __________ bridges using a blender.
a. transformation
b. transduction
c. conjugation
d. transposition
e. bacteriophage - (ANSWER)conjugation
A derivative F plasmid that contains host DNA is called an:
a. F plasmid
b. F+ plasmid
c. F- plasmid
d. F' plasmid
e. Hfr plasmid - (ANSWER)F' plasmid
Some conjugation systems use __________ to stimulate transfer of genetic information.
a. transformation
b. pheromones
c. transduction
d. transposition
e. reversion - (ANSWER)pheromones
Salmonella can conduct interspecies __________ with E. coli.
a. transduction
b. transformation
c. transposition
