1|Page
WGU D104 INTERMEDIATE ACCOUNTING II OA & PA
EXAM WITH COMPLETE 250 REAL EXAM QUESTIONS
AND CORRECT VERIFIED ANSWERS/ ALREADY GRADED
A+ LATEST UPDATE 2026 (MOST RECENT!!)
Which of the following gives rise to the skeletal and connective tissue of
the vertebrate limb form from:
a) the ectodermal epithelium of the limb bud
b) the mesodermal mesenchyme of the limb bud, derived from lateral
plate ectoderm
c) mesodermal cells that migrate into the limb bud from the somites
d) the progress zone
e) the polarizing region ......ANSWER......b) the mesodermal
mesenchyme of the limb bud, derived from lateral plate ectoderm
Where do the muscle-forming cells of the vertebrate limb come from:
a) the ectodermal epithelium of the limb bud
b) mesodermal cells that migrate from lateral plate ectoderm
c) mesodermal cells that migrate into the limb bud from the somites
d) the progress zone
e) the polarizing region ......ANSWER......c) mesodermal cells that
migrate into the limb bud from the somites
,2|Page
What is the way to demonstrate the importance of FGF signaling in
initiating limbdevelopment? ......ANSWER......Generate FGF receptor KO
mice and examine the limb phenotypes
Examine whether implantation of FGF-soaked beads can replace AER
activity
What is a transcription factor that specifies forelimb identify in
developing limbs. ......ANSWER......Tbx5
What is a growth factor that is responsible for the proximodistal axis
formation of the developing limbs? ......ANSWER......FGF8
The ____________ patterns the AP axis, and the AER maintains
outgrowth of the limb bud. ......ANSWER......ZPA
What is a growth factor that promotes apoptosis in the inter digit region
of the developingchick leg? ......ANSWER......BMP
__________ is the occurrence of extra digits on hands or feet, and often
caused by ectopicexpression of the _______ gene.
......ANSWER......Polydactyly, Shh or HoxD13
,3|Page
If ZPA from a wing is grafted into the anterior margin of a chick leg,
what will happen tothe transplanted limb?
......ANSWER......Transplanted tissue will induce extra toes
T/F: If ZPA of a leg bud is grafted into the anterior region of a wing bud,
the grafted limb bud will have two HoxD13 expression domains.
......ANSWER......TRUE
T/F: The developing limb buds of the embryo lacking engrailed result in
the conversion of the dorsal ectoderm towards the ventral ectoderm.
......ANSWER......FALSE
T/F: During limb development, the first mesodermal cells to exit the
progress zone will become proximal and the last will become more
distal structures. ......ANSWER......TRUE
T/F: The experiment using FGF8 soaked beads during limb outgrowth
shows that FGF8 is important in ZPA formation. ......ANSWER......FALSE
T/F: If a mouse forearm ZPA replaces the ZPA of chick wing bud, the
transplanted wing bud will become a normal mouse forearm.
......ANSWER......FALSE
Signals that regulate the growth and patterning of the limbs along the
anterior-posterior axis are:
, 4|Page
a) Signals from the neural tube.
b) Signals from the ZPA of the limb buds.
c) Signals in the ventral ectoderm of the limb buds. ......ANSWER......b)
Signals from the ZPA of the limb buds.
Transplantation of a small prospective thigh tissue fragment into the
distal tip of the wing bud has demonstrated
a) the thigh cells are not yet determined to become as leg or wing
b) signals that control limb patterning (specifying proximal/distal axis)
are equivalent
c) the thigh cells are irrevocably determined to become a particular part
of the leg ......ANSWER......b) signals that control limb patterning
(specifying proximal/distal axis) are equivalent
Which of the following signaling pathways directly controls the
sequence of limb digits across the antero-posterior axis?
a) FGF
b) Shh
WGU D104 INTERMEDIATE ACCOUNTING II OA & PA
EXAM WITH COMPLETE 250 REAL EXAM QUESTIONS
AND CORRECT VERIFIED ANSWERS/ ALREADY GRADED
A+ LATEST UPDATE 2026 (MOST RECENT!!)
Which of the following gives rise to the skeletal and connective tissue of
the vertebrate limb form from:
a) the ectodermal epithelium of the limb bud
b) the mesodermal mesenchyme of the limb bud, derived from lateral
plate ectoderm
c) mesodermal cells that migrate into the limb bud from the somites
d) the progress zone
e) the polarizing region ......ANSWER......b) the mesodermal
mesenchyme of the limb bud, derived from lateral plate ectoderm
Where do the muscle-forming cells of the vertebrate limb come from:
a) the ectodermal epithelium of the limb bud
b) mesodermal cells that migrate from lateral plate ectoderm
c) mesodermal cells that migrate into the limb bud from the somites
d) the progress zone
e) the polarizing region ......ANSWER......c) mesodermal cells that
migrate into the limb bud from the somites
,2|Page
What is the way to demonstrate the importance of FGF signaling in
initiating limbdevelopment? ......ANSWER......Generate FGF receptor KO
mice and examine the limb phenotypes
Examine whether implantation of FGF-soaked beads can replace AER
activity
What is a transcription factor that specifies forelimb identify in
developing limbs. ......ANSWER......Tbx5
What is a growth factor that is responsible for the proximodistal axis
formation of the developing limbs? ......ANSWER......FGF8
The ____________ patterns the AP axis, and the AER maintains
outgrowth of the limb bud. ......ANSWER......ZPA
What is a growth factor that promotes apoptosis in the inter digit region
of the developingchick leg? ......ANSWER......BMP
__________ is the occurrence of extra digits on hands or feet, and often
caused by ectopicexpression of the _______ gene.
......ANSWER......Polydactyly, Shh or HoxD13
,3|Page
If ZPA from a wing is grafted into the anterior margin of a chick leg,
what will happen tothe transplanted limb?
......ANSWER......Transplanted tissue will induce extra toes
T/F: If ZPA of a leg bud is grafted into the anterior region of a wing bud,
the grafted limb bud will have two HoxD13 expression domains.
......ANSWER......TRUE
T/F: The developing limb buds of the embryo lacking engrailed result in
the conversion of the dorsal ectoderm towards the ventral ectoderm.
......ANSWER......FALSE
T/F: During limb development, the first mesodermal cells to exit the
progress zone will become proximal and the last will become more
distal structures. ......ANSWER......TRUE
T/F: The experiment using FGF8 soaked beads during limb outgrowth
shows that FGF8 is important in ZPA formation. ......ANSWER......FALSE
T/F: If a mouse forearm ZPA replaces the ZPA of chick wing bud, the
transplanted wing bud will become a normal mouse forearm.
......ANSWER......FALSE
Signals that regulate the growth and patterning of the limbs along the
anterior-posterior axis are:
, 4|Page
a) Signals from the neural tube.
b) Signals from the ZPA of the limb buds.
c) Signals in the ventral ectoderm of the limb buds. ......ANSWER......b)
Signals from the ZPA of the limb buds.
Transplantation of a small prospective thigh tissue fragment into the
distal tip of the wing bud has demonstrated
a) the thigh cells are not yet determined to become as leg or wing
b) signals that control limb patterning (specifying proximal/distal axis)
are equivalent
c) the thigh cells are irrevocably determined to become a particular part
of the leg ......ANSWER......b) signals that control limb patterning
(specifying proximal/distal axis) are equivalent
Which of the following signaling pathways directly controls the
sequence of limb digits across the antero-posterior axis?
a) FGF
b) Shh
