MCB 104 Exam 1#1 Questions with correct answers
Type of microscopy: Can image live cells, but cannot label individual molecules -
✔✔Phase contrast Microscopy
Chromatin remodelers: what do they modify about chromatin - ✔✔Histone
location
Lamina - ✔✔Inner layer of the nuclear envelope, provides structural support.
Made of lamin proteins
Ran protein - ✔✔GTPase, controls binding and release of proteins across the
nuclear membrane via importins and exportins
Location of RanGAP - ✔✔Cytoplasm: GTPase activating protein: promotes
hydrolysis of GTP by Ran
Location of RanGEF - ✔✔Nucleus: Guanosine nucleotide exchange factor
(Replaces GDP with GTP on Ran protein)
Karyopherins - ✔✔importins and exportins for nuclear membrane transport
DamID - ✔✔Used to map sequences in proximity to a protein of interest. Dam
protein methylates adenosine in nearby DNA, which can be detected by
antibodies
, Indirect Immunofluorescence - ✔✔Uses a primary and secondary antibody to
allow target molecules to fluoresce. Allows for amplification of fluorescence
FRAP - ✔✔Fluorescence recovery after photobleaching
What type of amino acids and secondary structures do you find in the
membrane? - ✔✔Hydrophobic amino acids in alpha helices
Clathrin - ✔✔Protein that coats the internal surface of the plasma membrane and
provides part of the energy to bend the membrane for vesicle endocytosis
SRP - ✔✔Signal recognition particle: binds the ER signal sequence on actively
synthesizing proteins and halts translation and signals movement to the ER. Binds
SRP receptor on the ER membrane which feeds protein through a protein
translocator
What type of molecules can be added at what location to modify proteins to
associate more readily with membranes? - ✔✔Lipophilic molecules at the C-
terminus
Cis golgi network is closer to the ____ - ✔✔Endoplasmic reticulum
Rab protein - ✔✔Small GTP-binding protein bound to vesicles in the cytoplasm,
which help target them to tethering proteins. T-snares and V-snares assist with
vesicle fusion
Actin bound to ATP more readily... - ✔✔Incorporates into filaments
Type of microscopy: Can image live cells, but cannot label individual molecules -
✔✔Phase contrast Microscopy
Chromatin remodelers: what do they modify about chromatin - ✔✔Histone
location
Lamina - ✔✔Inner layer of the nuclear envelope, provides structural support.
Made of lamin proteins
Ran protein - ✔✔GTPase, controls binding and release of proteins across the
nuclear membrane via importins and exportins
Location of RanGAP - ✔✔Cytoplasm: GTPase activating protein: promotes
hydrolysis of GTP by Ran
Location of RanGEF - ✔✔Nucleus: Guanosine nucleotide exchange factor
(Replaces GDP with GTP on Ran protein)
Karyopherins - ✔✔importins and exportins for nuclear membrane transport
DamID - ✔✔Used to map sequences in proximity to a protein of interest. Dam
protein methylates adenosine in nearby DNA, which can be detected by
antibodies
, Indirect Immunofluorescence - ✔✔Uses a primary and secondary antibody to
allow target molecules to fluoresce. Allows for amplification of fluorescence
FRAP - ✔✔Fluorescence recovery after photobleaching
What type of amino acids and secondary structures do you find in the
membrane? - ✔✔Hydrophobic amino acids in alpha helices
Clathrin - ✔✔Protein that coats the internal surface of the plasma membrane and
provides part of the energy to bend the membrane for vesicle endocytosis
SRP - ✔✔Signal recognition particle: binds the ER signal sequence on actively
synthesizing proteins and halts translation and signals movement to the ER. Binds
SRP receptor on the ER membrane which feeds protein through a protein
translocator
What type of molecules can be added at what location to modify proteins to
associate more readily with membranes? - ✔✔Lipophilic molecules at the C-
terminus
Cis golgi network is closer to the ____ - ✔✔Endoplasmic reticulum
Rab protein - ✔✔Small GTP-binding protein bound to vesicles in the cytoplasm,
which help target them to tethering proteins. T-snares and V-snares assist with
vesicle fusion
Actin bound to ATP more readily... - ✔✔Incorporates into filaments
