BIOL 205 EXAM 1 QUESTIONS AND
VERIFIED ANSWERS (LATEST 2026-
2027) 100% CORRECT
4 methods to homogenize cells - correct answer-
sonication: high frequency sound lyses cells
mechanical force: shear cells (plant cells)
detergent: lyses cells by put holes in the plasma
membrane
pressure: cells forced through a small hole using high
pressure break open
active transport - correct answer- form of
transport that moves solutes across membrane against
their concentration gradient. carried out by pumps that
harvest energy from atp, ion gradient, or ligh
adaptin - correct answer- ecure the clathrin
coat to the vesicle membrane and help select cargo
molecules for transport by trapping cargo receptors
,affinity chromatography - correct answer-
beads are coated w/ a receptor that binds the protein of
interest (high affinity)
- protein is retained in column
-eluted also by changing pH or salinity levels to disrupt
bond b/w ligand & protein
- can be eluted by washing the column w/ a free receptor
that competes w/ the bead bound receptor & frees
protein
alpha-helix - correct answer- is generated when
a single polypeptide chain turns around itself to form a
structurally rigid cylinder. A hydrogen bond is made
between every fourth amino acid, linking the C=O of one
peptide bond to the N-H of another
amino acid polar/nonpolar chart - correct answer-
polar = hydrophilic
,non-polar=hydrophobic
amphipathic molecule - correct answer- has
both hydrophobic + hydrophilic region
animal cell - correct answer-
antibody (fluorescent tool) - correct answer-
conjugates to a fluorophore that recognize a protein that
is abundant in and localized to the area
antiporter - correct answer- A carrier protein
that transports two molecules acrss the plasma
membrane in opposite directions.
Are virsuses alive? - correct answer- no,
because they do no reproduce on their own
ATP-driven pumps - correct answer- hydrolyze
ATP to drive uphill transport.
, Autophagy - correct answer- A process in which
lysosomes decompose damaged organelles to reuse their
organic monomers
Backbone model of protein - correct answer-
shows overall organization of the protein making it easy
to compare protein of interest to other proteins
beta-sheet - correct answer- made when
hydrogen bonds form between segments of a polypeptide
chain that lie side by side
When the neighboring segments run in the same
orientation (say, from the N-terminus to the C-terminus),
the structure is a parallel β sheet; when they run in
opposite directions, the structure is an antiparallel β
sheet.
BiFC - correct answer- figuring out if proteins
interact; if they interact, GFP combines and glows
VERIFIED ANSWERS (LATEST 2026-
2027) 100% CORRECT
4 methods to homogenize cells - correct answer-
sonication: high frequency sound lyses cells
mechanical force: shear cells (plant cells)
detergent: lyses cells by put holes in the plasma
membrane
pressure: cells forced through a small hole using high
pressure break open
active transport - correct answer- form of
transport that moves solutes across membrane against
their concentration gradient. carried out by pumps that
harvest energy from atp, ion gradient, or ligh
adaptin - correct answer- ecure the clathrin
coat to the vesicle membrane and help select cargo
molecules for transport by trapping cargo receptors
,affinity chromatography - correct answer-
beads are coated w/ a receptor that binds the protein of
interest (high affinity)
- protein is retained in column
-eluted also by changing pH or salinity levels to disrupt
bond b/w ligand & protein
- can be eluted by washing the column w/ a free receptor
that competes w/ the bead bound receptor & frees
protein
alpha-helix - correct answer- is generated when
a single polypeptide chain turns around itself to form a
structurally rigid cylinder. A hydrogen bond is made
between every fourth amino acid, linking the C=O of one
peptide bond to the N-H of another
amino acid polar/nonpolar chart - correct answer-
polar = hydrophilic
,non-polar=hydrophobic
amphipathic molecule - correct answer- has
both hydrophobic + hydrophilic region
animal cell - correct answer-
antibody (fluorescent tool) - correct answer-
conjugates to a fluorophore that recognize a protein that
is abundant in and localized to the area
antiporter - correct answer- A carrier protein
that transports two molecules acrss the plasma
membrane in opposite directions.
Are virsuses alive? - correct answer- no,
because they do no reproduce on their own
ATP-driven pumps - correct answer- hydrolyze
ATP to drive uphill transport.
, Autophagy - correct answer- A process in which
lysosomes decompose damaged organelles to reuse their
organic monomers
Backbone model of protein - correct answer-
shows overall organization of the protein making it easy
to compare protein of interest to other proteins
beta-sheet - correct answer- made when
hydrogen bonds form between segments of a polypeptide
chain that lie side by side
When the neighboring segments run in the same
orientation (say, from the N-terminus to the C-terminus),
the structure is a parallel β sheet; when they run in
opposite directions, the structure is an antiparallel β
sheet.
BiFC - correct answer- figuring out if proteins
interact; if they interact, GFP combines and glows
