HK 100 EXAM 2 FINAL PAPER TEST BANK WITH VERIFIED QUESTIONS AND DETAILED SOLUTIONS

HK 100 EXAM 2 FINAL PAPER TEST BANK
WITH VERIFIED QUESTIONS AND
DETAILED SOLUTIONS

●● Michaelis-Menten equation
Answer: V = (Vmax[S]) / (Km + [S])


●● Km definition
Answer: Substrate concentration at 1/2 Vmax


●● Km meaning
Answer: Inversely related to enzyme affinity (low Km high affinity)


●● Vmax definition
Answer: Maximum velocity when enzyme is saturated


●● At substrate equals Km
Answer: Reaction rate is 1/2 Vmax


●● Lineweaver-Burk equation
Answer: 1/V = (Km/Vmax)(1/[S]) + 1/Vmax

, ●● Lineweaver-Burk y intercept
Answer: 1/Vmax


●● Lineweaver-Burk x intercept
Answer: -1/Km


●● Lineweaver-Burk slope
Answer: Km/Vmax


●● Competitive inhibition
Answer: Inhibitor binds active site increases Km does not change Vmax


●● Noncompetitive inhibition
Answer: Inhibitor binds away from active site decreases Vmax does not
change Km


●● Irreversible inhibition
Answer: Covalent binding permanently inactivates enzyme


●● Competitive inhibition effect at high substrate
Answer: Can be overcome by increasing substrate concentration