Portage Microbiology 271: Module 3 Questions With
Complete Solutions
objective lens
lens closest to the sample; greatest amount of
magnification (more light = more magnification degree)
ocular lens
eyepiece for the eye; (10x)
multicaptive
when ocular and objective lens' are used together
(10x + 40x = 400x magnification)
what is required before staining?
heat or chemical methods
Phase Contrast: uses
1. view internal structures of live specimen
2. observe motility (cilia flagella)
specialized
Phase Contrast: features
no staining necessary so we can view LIVE specimens
Phase Contrast: images
show light and dark areas of microbe
, Phase Contrast: advantages
over bright > able to visualize invisible structures without
staining.
Dark field: uses
view living and unstained specimens
Dark field: features
filter inhibits light from going through organism and instead
light is reflected by organism
Dark field: images
dark background with bright specimen
Fluorescence: uses
1. localize specific structures or molecules
2. diagnostic tool
Fluorescence: features
uses UV light to excite flurophores
whole cells, specific structures or proteins and watch movement
or interactions
Fluorescence: images
dark background with florescent structures
aura amoenoe
stain used
Immunofluorescence: uses
Complete Solutions
objective lens
lens closest to the sample; greatest amount of
magnification (more light = more magnification degree)
ocular lens
eyepiece for the eye; (10x)
multicaptive
when ocular and objective lens' are used together
(10x + 40x = 400x magnification)
what is required before staining?
heat or chemical methods
Phase Contrast: uses
1. view internal structures of live specimen
2. observe motility (cilia flagella)
specialized
Phase Contrast: features
no staining necessary so we can view LIVE specimens
Phase Contrast: images
show light and dark areas of microbe
, Phase Contrast: advantages
over bright > able to visualize invisible structures without
staining.
Dark field: uses
view living and unstained specimens
Dark field: features
filter inhibits light from going through organism and instead
light is reflected by organism
Dark field: images
dark background with bright specimen
Fluorescence: uses
1. localize specific structures or molecules
2. diagnostic tool
Fluorescence: features
uses UV light to excite flurophores
whole cells, specific structures or proteins and watch movement
or interactions
Fluorescence: images
dark background with florescent structures
aura amoenoe
stain used
Immunofluorescence: uses
