Micro 020 Final Lab Practicum Actual EXAM
Questions And CORRECT Answers
List the steps of the aseptic technique - CORRECT ANSWERS 1. Flame the loop to sterilize it
2. Remove the tube cap
3. Flame tube top to sterilize it
4. Stick sterilized portion of the loop into the tube
5. Reflame tube
6. Recap tube. Reflame loop to sterilize it
Explain the process of quadrant streaking - CORRECT ANSWERS 1. Sterilize
2. Collect 1 loop of sample
3. Streak first quadrant from perimeter to just above center
3. 2nd streak should slightly pass through the first
4. 3rd streak should slightly pass through the second only
5. 4th streak should slightly pass through the 3rd only from top to full center *Do not pass through
first quadrant or down into 2nd quadrant
What are the bacterium counts per quadrant (Quadrant Streak)? - CORRECT
ANSWERS Greatest to least
1st: Millions, billions
2nd: 10^4 - 10^7
3rd: 10^2 - 10^3
4th: 1 - 50
List all microscope parts - CORRECT ANSWERS 1. Eye piece: View bacterium
2. Tube: Connects to eye piece, light travels through it
3. Nose: Above lenses
4. Objective lenses: Self
5. Stage clip
6. Stage: Slides rest on it
, 7. Arm: Holds optical pieces and lenses
8. Course/Fine adjustment knobs: for viewing bacterium clearly
9. Coaxial stage controls (up/down)
10. Light Source
11. Base: Self
Explain biochemical test: MSA - CORRECT ANSWERS Media Salt Agar
To see if bacteria can ferment mannitol
(+) result:
(-) result:
Strong: Staph
Explain biochemical test: MAC (MacConkey) - CORRECT ANSWERS MacConkey Agar
Identify Lactose fermenting bacteria
(+) result: Bright pink/red
(-) no color
Explain biochemical test: EMB - CORRECT ANSWERS Eosin Methylene Blue
Differentiate bacteria / To see if bacteria ferments sugars to produce acids for visual response
(+) strong: Green metalic
(+) less strong: dark purple/black
(-) Pink
Explain biochemical test: Blood Hemolysis - CORRECT ANSWERS Isolate + differentiate bacteria
that produce hemolysins (Breaks down RBCs)
Questions And CORRECT Answers
List the steps of the aseptic technique - CORRECT ANSWERS 1. Flame the loop to sterilize it
2. Remove the tube cap
3. Flame tube top to sterilize it
4. Stick sterilized portion of the loop into the tube
5. Reflame tube
6. Recap tube. Reflame loop to sterilize it
Explain the process of quadrant streaking - CORRECT ANSWERS 1. Sterilize
2. Collect 1 loop of sample
3. Streak first quadrant from perimeter to just above center
3. 2nd streak should slightly pass through the first
4. 3rd streak should slightly pass through the second only
5. 4th streak should slightly pass through the 3rd only from top to full center *Do not pass through
first quadrant or down into 2nd quadrant
What are the bacterium counts per quadrant (Quadrant Streak)? - CORRECT
ANSWERS Greatest to least
1st: Millions, billions
2nd: 10^4 - 10^7
3rd: 10^2 - 10^3
4th: 1 - 50
List all microscope parts - CORRECT ANSWERS 1. Eye piece: View bacterium
2. Tube: Connects to eye piece, light travels through it
3. Nose: Above lenses
4. Objective lenses: Self
5. Stage clip
6. Stage: Slides rest on it
, 7. Arm: Holds optical pieces and lenses
8. Course/Fine adjustment knobs: for viewing bacterium clearly
9. Coaxial stage controls (up/down)
10. Light Source
11. Base: Self
Explain biochemical test: MSA - CORRECT ANSWERS Media Salt Agar
To see if bacteria can ferment mannitol
(+) result:
(-) result:
Strong: Staph
Explain biochemical test: MAC (MacConkey) - CORRECT ANSWERS MacConkey Agar
Identify Lactose fermenting bacteria
(+) result: Bright pink/red
(-) no color
Explain biochemical test: EMB - CORRECT ANSWERS Eosin Methylene Blue
Differentiate bacteria / To see if bacteria ferments sugars to produce acids for visual response
(+) strong: Green metalic
(+) less strong: dark purple/black
(-) Pink
Explain biochemical test: Blood Hemolysis - CORRECT ANSWERS Isolate + differentiate bacteria
that produce hemolysins (Breaks down RBCs)
