BSCI 1510 FINAL EXAM
BSCI 1510 FINAL EXAM QUESTIONS AND ANSWERS GRADED A+
Which way is RNA synthesized? ANS >> 5' to 3'
Net yield of glycolysis ANS >> 2 ATPs, 2 NADHs, and 2
pyruvates
What components are required for transcription?
ANS >> RNA polymerase, DNA template,
ribonucleotide triphosphates, and Mg2+
Reactants of glycolysis ANS >> 2 ATPS, one glucose
molecule
,BSCI 1510 FINAL EXAM
Promoter in prokaryotes ANS >> region of DNA that
indicates to an enzyme where to bind to make RNA,
located at -10, -35
Is oxygen required for glycolysis? ANS >> NO
sigma factor ANS >> -a protein that associates with
RNA polymerase that facilitates its binding to
specific promoters
-recognizes the Prin bow box
Step 1 of Glycolysis Mechanism (need to know all
components) ANS >> -Glucose is phosphorylated into
glucose 6-phosphate by hexokinase
-ATP is converted to ADP
-irreversible
Where do Raps start transcription? ANS >> +1,
directly upstream of the promoter region
,BSCI 1510 FINAL EXAM
Step 2 of Glycolysis Mechanism (just reactant and
product) ANS >> -Glucose 6-phosphate is converted to
fructose 6-phosphate by an isomerase
-readily reversible
Step 3 of Glycolysis Mechanism (need to know all
components) ANS >> -fructose 6-phosphate is
phosphorylated into fructose 1,6-biphosphate by
phosphofructokinase
-ATP is converted to ADP
-Major regulated step
-irreversible
What are the types of prokaryotic RNA chain
termination? ANS >> Rho dependent and rho
independent termination
, BSCI 1510 FINAL EXAM
Steps 4 and 5 of Glycolysis general mechanism
(initial reactant and final product) ANS >> Fructose 1,6
biphosphate (6 carbon sugar) is split into two
glyceraldehyde 3-phosphate (3 carbon sugar)
-readily reversible
Rho-dependent termination ANS >> Rho binds to RNA
and when it catches up to RNAP, it causes it to fall
off
In what conditions can Rho catch up to RNAP? ANS >>
When a hairpin forms or when there are low levels
of nucleotides
Steps 6 and 7 of Glycolysis general mechanism (just
know products) ANS >> -Harvest 2 ATPs and 1 NADH
-readily reversible
BSCI 1510 FINAL EXAM QUESTIONS AND ANSWERS GRADED A+
Which way is RNA synthesized? ANS >> 5' to 3'
Net yield of glycolysis ANS >> 2 ATPs, 2 NADHs, and 2
pyruvates
What components are required for transcription?
ANS >> RNA polymerase, DNA template,
ribonucleotide triphosphates, and Mg2+
Reactants of glycolysis ANS >> 2 ATPS, one glucose
molecule
,BSCI 1510 FINAL EXAM
Promoter in prokaryotes ANS >> region of DNA that
indicates to an enzyme where to bind to make RNA,
located at -10, -35
Is oxygen required for glycolysis? ANS >> NO
sigma factor ANS >> -a protein that associates with
RNA polymerase that facilitates its binding to
specific promoters
-recognizes the Prin bow box
Step 1 of Glycolysis Mechanism (need to know all
components) ANS >> -Glucose is phosphorylated into
glucose 6-phosphate by hexokinase
-ATP is converted to ADP
-irreversible
Where do Raps start transcription? ANS >> +1,
directly upstream of the promoter region
,BSCI 1510 FINAL EXAM
Step 2 of Glycolysis Mechanism (just reactant and
product) ANS >> -Glucose 6-phosphate is converted to
fructose 6-phosphate by an isomerase
-readily reversible
Step 3 of Glycolysis Mechanism (need to know all
components) ANS >> -fructose 6-phosphate is
phosphorylated into fructose 1,6-biphosphate by
phosphofructokinase
-ATP is converted to ADP
-Major regulated step
-irreversible
What are the types of prokaryotic RNA chain
termination? ANS >> Rho dependent and rho
independent termination
, BSCI 1510 FINAL EXAM
Steps 4 and 5 of Glycolysis general mechanism
(initial reactant and final product) ANS >> Fructose 1,6
biphosphate (6 carbon sugar) is split into two
glyceraldehyde 3-phosphate (3 carbon sugar)
-readily reversible
Rho-dependent termination ANS >> Rho binds to RNA
and when it catches up to RNAP, it causes it to fall
off
In what conditions can Rho catch up to RNAP? ANS >>
When a hairpin forms or when there are low levels
of nucleotides
Steps 6 and 7 of Glycolysis general mechanism (just
know products) ANS >> -Harvest 2 ATPs and 1 NADH
-readily reversible
