BIO 250 MICROBIOLOGY LAB 1 2026 STUDY GUIDE COMPLETE EXPERIMENT QUESTIONS AND ANSWERS 100% CORRECT

BIO 250 MICROBIOLOGY LAB 1 2026 STUDY
GUIDE COMPLETE EXPERIMENT QUESTIONS
AND ANSWERS 100% CORRECT

◉ How many colonies are on a countable plate. Answer: between
30-300 CFUs


◉ NON-LACTOSE FERMENTERS on the EMB and McConkey agars
appear ____________________.. Answer: Colorless


◉ On EMB agar, LACTOSE FERMENTERS appear ____________________
(small and large acid production). Answer: Metallic Green for large
acid production Purple for small acid production


◉ Beta-hemolysis (complete cell lysis). Answer: Clearing around
growth


◉ Alpha-hemolysis (partial cell lysis). Answer: Greening around
growth


◉ Gamma-hemolysis (no cell lysis). Answer: No change in the
medium

, ◉ Positive Result For Catalase Test. Answer: Bubbles


◉ Negative Result For Catalase Test. Answer: No Bubbles


◉ Positive Result For Coagulase Test. Answer: Plasma forms a clot


◉ Negative Result For Coagulase Test. Answer: Plasma remains
liquid


◉ Positive Result For Gelatin Liquidification Test. Answer: Gelatin
Liquified


◉ Negative Result For Gelatin Liquidification Test. Answer: Gelatin
remained solid


◉ What is the importance of the Coagulase Test?. Answer: It
distinguishes Staphylococcus aureus from other Gram positive cocci


◉ What is the enzyme used in the Gelatin Liquidification Test?.
Answer: Gelatinase


◉ Positive Result For Hydrolysis of Milk Protein Test. Answer:
clearing around bacteria