ASCP CERTIFICATION EXAM QUESTIONS
AND ANSWERS UPDATED 2026-2027
# Term Definition
1 4 heme, 4 iron, 1 globin
What is hemoglobin composed of?
2 porphyria
What disorder does defect in heme
synthesis lead to?
3 to keep iron in ferrous state
What is the function of the enzyme
methemoglobin-cytochrome C
reductase in red blood cell?
4 carboxyhemoglobin
Which hemoglobin has a 200 times
greater binding capacity for the
hemoglobin molecule than oxygen?
5 methemoglobin
What product is formed when ferrous
iron of hemoglobin is oxidized to the
ferric state?
6 sulfhemoglobin
Which hemoglobin derivative causes
irreversible changes in the red blood
cells?
7 Hb-S
Which of the following hemoglobins is
NOT normally present in a normal
adult?
8 Hb-F
Which embryonic hemoglobin is
normally found in newborns?
9 Hb-F
What is the most abundant hemoglobin
in a newborn?
, 10
What is the molecular structure of valine replaces gultamic acid in the 6th position of the
hemoglobin S? beta chain
11 Hb-S
Which of the following hemoglobins is
insoluble under lowered oxygen
tension?
# Term Definition
12 hemoglobin electrophoresis
What laboratory test is used to confirm
the specific diagnosis of sickle cell
anemia?
13 sodium dithionite
In the solubility test for hemoglobin S,
what is the reagent responsible for the
reduction of hemoglobin molecule?
14 lysine
In hemoglobin C, what replaces
glutamic acid?
15 rod-shaped crystals
Which red blood cell inclusion is
characteristic of hemoglobin C disease?
16 2 alpha + 2 gamma chains
What is hemoglobin F composed of?
17 Hb-F
Which hemoglobin is resistant to alkali
denaturation?
18 Hb-A1 from Hb-F
What hemoglobins are differentiated
by the
Kleihauer-Betke technique?
AND ANSWERS UPDATED 2026-2027
# Term Definition
1 4 heme, 4 iron, 1 globin
What is hemoglobin composed of?
2 porphyria
What disorder does defect in heme
synthesis lead to?
3 to keep iron in ferrous state
What is the function of the enzyme
methemoglobin-cytochrome C
reductase in red blood cell?
4 carboxyhemoglobin
Which hemoglobin has a 200 times
greater binding capacity for the
hemoglobin molecule than oxygen?
5 methemoglobin
What product is formed when ferrous
iron of hemoglobin is oxidized to the
ferric state?
6 sulfhemoglobin
Which hemoglobin derivative causes
irreversible changes in the red blood
cells?
7 Hb-S
Which of the following hemoglobins is
NOT normally present in a normal
adult?
8 Hb-F
Which embryonic hemoglobin is
normally found in newborns?
9 Hb-F
What is the most abundant hemoglobin
in a newborn?
, 10
What is the molecular structure of valine replaces gultamic acid in the 6th position of the
hemoglobin S? beta chain
11 Hb-S
Which of the following hemoglobins is
insoluble under lowered oxygen
tension?
# Term Definition
12 hemoglobin electrophoresis
What laboratory test is used to confirm
the specific diagnosis of sickle cell
anemia?
13 sodium dithionite
In the solubility test for hemoglobin S,
what is the reagent responsible for the
reduction of hemoglobin molecule?
14 lysine
In hemoglobin C, what replaces
glutamic acid?
15 rod-shaped crystals
Which red blood cell inclusion is
characteristic of hemoglobin C disease?
16 2 alpha + 2 gamma chains
What is hemoglobin F composed of?
17 Hb-F
Which hemoglobin is resistant to alkali
denaturation?
18 Hb-A1 from Hb-F
What hemoglobins are differentiated
by the
Kleihauer-Betke technique?
