UAMS HEMATOLOGY LAB FINAL EXAM
WITH CORRECT ACTUAL QUESTIONS AND
CORRECTLY WELL DEFINED ANSWERS
LATEST ALREADY GRADED A+
What happens if the cleaning solution doesn't dry on the
hemacytometer?
The red cells will cause cells to lyse.
How do you make a 1:20 dilution of acetic acid and whole
blood when performing a manual WBC count?
Add 475 uL of 3% acetic acid. Add 25 uL of well-mixed whole
blood to the tube. Swirl to mix. Allow the dilution to sit for 10
minutes to lyse the red cells.
What are the dimensions to the hemacytometer?
Grid Dimensions
Each large square is 1mm
The entire width is 3mm
The entire height is 3mm
Grid area: 9mm2
,Depth (distant between cover glass and grid): 0.1mm
Total volume: 9mm2 x o.1mm= 0.9mm3
What is the smear prep process?
Place drop of blood from EDTA tube on with 1/2 inch from
the frosted end of a slide. Lay the slide on a flat surface. Place
the end of a second (spreader) slide, held with the fingertips
at an angle of 30-45 degrees, in front of the drop of blood.
Pull the spreader slide back into the drop of blood. Allow
blood to spread entire width of slide. Quickly and smoothly
push the spreader slide forward.
What tools do you need to prepare a smear?
Glass slides, blood, cover slip
What causes a bad smear?
Dirty slide, jerky motion, pointed feather edge, angle of the
spreader slide too steep, did not allow blood to spread, high
level of lipids, unequal pressure.
What is the coverslip smear used for?
Used for making bone marrow preps.
What the items needed for the Wright's stain?
Wright's stain, buffer, rinse solution, timer, jars or other
containers for holding the stain, buffer, and rinse.
What are the sources of error when staining a smear?
, Stain or buffer is too acidic or alkaline, prolonged staining,
heparinized blood sample, prolonged rinsing, under-
buffering.
What is a good area when viewing a blood smear?
Cells should be touching each other with minimal overlap.
How do you perform a WBC estimate?
Select a good area on slide. Average the number of white
cells in five low power fields. Multiply the average by 200.
(One white cell under low power, 10x, equals approximately
200 WBCs)
How do you check for platelet clumps?
Check for platelet clumps after performing WBC estimate.
Look at the feather edge or side margins of the smear.
If you can't get your slide to focus, what are the potential
issues?
The slide is upside down. A drop of oil wasn't added to the
slide. The coarse adjust knob has been turned too far one
way or another.
What is a good area in oil immersion?
Cells touching with minimal overlap. Approximately 200 RBCs
per oil field.
What do we look at under oil immersion?
WITH CORRECT ACTUAL QUESTIONS AND
CORRECTLY WELL DEFINED ANSWERS
LATEST ALREADY GRADED A+
What happens if the cleaning solution doesn't dry on the
hemacytometer?
The red cells will cause cells to lyse.
How do you make a 1:20 dilution of acetic acid and whole
blood when performing a manual WBC count?
Add 475 uL of 3% acetic acid. Add 25 uL of well-mixed whole
blood to the tube. Swirl to mix. Allow the dilution to sit for 10
minutes to lyse the red cells.
What are the dimensions to the hemacytometer?
Grid Dimensions
Each large square is 1mm
The entire width is 3mm
The entire height is 3mm
Grid area: 9mm2
,Depth (distant between cover glass and grid): 0.1mm
Total volume: 9mm2 x o.1mm= 0.9mm3
What is the smear prep process?
Place drop of blood from EDTA tube on with 1/2 inch from
the frosted end of a slide. Lay the slide on a flat surface. Place
the end of a second (spreader) slide, held with the fingertips
at an angle of 30-45 degrees, in front of the drop of blood.
Pull the spreader slide back into the drop of blood. Allow
blood to spread entire width of slide. Quickly and smoothly
push the spreader slide forward.
What tools do you need to prepare a smear?
Glass slides, blood, cover slip
What causes a bad smear?
Dirty slide, jerky motion, pointed feather edge, angle of the
spreader slide too steep, did not allow blood to spread, high
level of lipids, unequal pressure.
What is the coverslip smear used for?
Used for making bone marrow preps.
What the items needed for the Wright's stain?
Wright's stain, buffer, rinse solution, timer, jars or other
containers for holding the stain, buffer, and rinse.
What are the sources of error when staining a smear?
, Stain or buffer is too acidic or alkaline, prolonged staining,
heparinized blood sample, prolonged rinsing, under-
buffering.
What is a good area when viewing a blood smear?
Cells should be touching each other with minimal overlap.
How do you perform a WBC estimate?
Select a good area on slide. Average the number of white
cells in five low power fields. Multiply the average by 200.
(One white cell under low power, 10x, equals approximately
200 WBCs)
How do you check for platelet clumps?
Check for platelet clumps after performing WBC estimate.
Look at the feather edge or side margins of the smear.
If you can't get your slide to focus, what are the potential
issues?
The slide is upside down. A drop of oil wasn't added to the
slide. The coarse adjust knob has been turned too far one
way or another.
What is a good area in oil immersion?
Cells touching with minimal overlap. Approximately 200 RBCs
per oil field.
What do we look at under oil immersion?
