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Study Guide for ACSM, ISSN, CSCS Exam
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Description:
Master sports nutrition for 2026-2027 with 100 exam-style questions on carbohydrate
loading, hydration protocols, protein requirements for athletes, vitamin and mineral
deficiencies, and energy systems.
Includes detailed answer explanations updated to current ISSN and ACSM position stands.
Download the complete practice exam to boost your CSCS, CISSN, or registered dietitian exam
score today.
, Sports Nutrition Exam 2026-2027: 100 Questions
Section A: Carbohydrate Loading and Muscle Glycogen Storage
Question 1
Consider an athlete completing a single-day carbohydrate loading strategy (no exercise
combined with 10g/kg body mass in the day prior to a competition). The athlete has a mass of
80kg. If 75% of the consumed carbohydrate is stored in muscles as glycogen, how much water
will have moved into the muscle cells as a result?
A) 0.9 kg
B) 1.2 kg
C) 1.8 kg
D) 2.4 kg
Answer: C
Explanation: Each gram of glycogen stored in muscle binds approximately 3-4 grams of water.
The athlete consumes 800g carbohydrate (80kg × 10g/kg). At 75% storage efficiency, 600g is
stored as glycogen (800g × 0.75). With a conservative estimate of 3g water per gram of
glycogen, 600g × 3 = 1800g or 1.8kg of water moves into muscle cells.
Section B: Hydration and Fluid Balance
Question 2
Which of the following is true regarding post-exercise hydration?
A) Athletes should consume 1 cup of fluid for every pound lost during exercise
B) Athletes should consume 2 cups of fluid for every pound that is lost during exercise
C) Athletes should consume 3 cups of fluid for every pound lost during exercise
D) Athletes should avoid fluid consumption immediately after exercise
Answer: B
,Explanation: Current sports nutrition guidelines recommend consuming approximately 16-24
ounces (2-3 cups) of fluid for every pound of body weight lost during exercise to ensure
adequate rehydration, accounting for ongoing urinary losses.
Question 3
Optimal hydration status should stimulate urination approximately every:
A) 30-60 minutes
B) 1-2 hours
C) 3-4 hours
D) 5-6 hours
Answer: B
Explanation: Well-hydrated individuals typically produce urine every 1-2 hours, with urine
appearing pale yellow to clear in color. Longer intervals between urination may indicate
inadequate hydration status.
Question 4
True or False: Body water is a poor heat conductor.
Answer: False
Explanation: Body water is an excellent heat conductor, which facilitates the transfer of heat
from deeper body tissues to the skin surface, enabling effective thermoregulation through
evaporative cooling.
Question 5
Which of the following can be sources of water for athletes needing to replenish fluid losses?
A) Beverages including water and sports drinks
B) Fruits and vegetables with high water content
C) Metabolic water produced during cellular respiration
D) All of the above
, Answer: D
Explanation: Athletes obtain water from multiple sources: fluids consumed, moisture in foods
(particularly fruits and vegetables which may contain 85-95% water), and metabolic water
produced as a byproduct of macronutrient oxidation.
Question 6
Which of the following conditions would inform an athlete that they may be dehydrated if they
are self-monitoring hydration status?
A) Urinating every 2 hours
B) Urinating every 4 hours
C) Pale yellow urine
D) Clear urine
Answer: B
Explanation: Urinating only every 4 hours or less frequently, combined with dark yellow or
amber-colored urine, is a practical self-assessment indicator of possible dehydration requiring
immediate fluid replenishment.
Question 7
Why is water arguably the most essential nutrient for athletes?
A) Water provides the most energy of any nutrient
B) Death occurs more rapidly in water's absence than in the absence of any other nutrient, and
muscle tissue is roughly 70% water
C) Water contains essential vitamins and minerals
D) Water is the only nutrient that builds muscle tissue
Answer: B
Explanation: Humans can survive weeks without food but only days without water.
Additionally, muscle tissue contains approximately 70-75% water by weight, highlighting
water's critical role in maintaining muscle function and overall physiological processes.