BIS 102 FINAL EXAM QUESTIONS AND
CORRECT DETAILED ANSWERS
a-helix - correct answer- no glycine, proline, serine
no +/+ or -/- electrostatic repulsion
no W-W or F-F
affinity chromatography - correct answer- - beads are
coated w/ a receptor that binds the protein of interest (high
affinity)
- protein is retained in column
- can be eluted by washing the column w/ a free receptor that
competes w/ the bead bound receptor & frees protein
-eluted also by changing pH/salinity levels to disrupt bond
between ligand & protein
allosteric effectors of Hb - correct answer- 1. CO2
2. H+
, 3. 2,3-BPG
4. Cl-
will cause a right shift in graph and favor deoxy Hb
ammonium sulfate fractionation - correct answer- when
added to a protein solution at high concentrations, it disrupts
the hydration shell around protein molecules by competing for
water molecule
salts out the protein and causes it to aggregate/precipitate
B (amino acid analysis) - correct answer- N (Asparagine)
--> D (Aspartic Acid) + NH4+
B-pleated sheet - correct answer- polypeptide chain link
side by side
Can D (aspartic acid) and E (glutamic acid) act as a H-bond
donor/acceptor? - correct answer- can only act as a
hydrogen bond acceptor
contains a carboxyl group with a (-)oxygen that can't donate H+
CORRECT DETAILED ANSWERS
a-helix - correct answer- no glycine, proline, serine
no +/+ or -/- electrostatic repulsion
no W-W or F-F
affinity chromatography - correct answer- - beads are
coated w/ a receptor that binds the protein of interest (high
affinity)
- protein is retained in column
- can be eluted by washing the column w/ a free receptor that
competes w/ the bead bound receptor & frees protein
-eluted also by changing pH/salinity levels to disrupt bond
between ligand & protein
allosteric effectors of Hb - correct answer- 1. CO2
2. H+
, 3. 2,3-BPG
4. Cl-
will cause a right shift in graph and favor deoxy Hb
ammonium sulfate fractionation - correct answer- when
added to a protein solution at high concentrations, it disrupts
the hydration shell around protein molecules by competing for
water molecule
salts out the protein and causes it to aggregate/precipitate
B (amino acid analysis) - correct answer- N (Asparagine)
--> D (Aspartic Acid) + NH4+
B-pleated sheet - correct answer- polypeptide chain link
side by side
Can D (aspartic acid) and E (glutamic acid) act as a H-bond
donor/acceptor? - correct answer- can only act as a
hydrogen bond acceptor
contains a carboxyl group with a (-)oxygen that can't donate H+
