BIOL 350 FINAL EXAM COMPLETE
QUESTIONS AND VERIFIED ANSWERS
\.How many domains of life are there? - ANSWERS✔-3: Bacteria; Archaea; Eukarya
\.Smallest taxonomic rank is __________. - ANSWERS✔-strain (species)
\.Largest taxonomic rank is __________. - ANSWERS✔-Domain
\.How many kingdoms are microorganisms found in? - ANSWERS✔-4
\.Genus and species are specific to _______ microorganism. - ANSWERS✔-one
\.Sequence alignment for DNA - ANSWERS✔-referenced as a percent identity,
higher the percent, higher the similarity between sequences
\.What type of genetic material do we use to identify bacteria and archaea? -
ANSWERS✔-16S rRNA
\.whole genome sequencing - ANSWERS✔-complete genetic information,
considered same species if 95% identity, can determine strains
, \.In order to extract DNA from a microbe we must _________. - ANSWERS✔-
culture the microbe
\.metagenomic sequencing - ANSWERS✔-DNA extracted from raw sample and
sequenced, identification is only as good as your database
\.strain - ANSWERS✔-an isolate that has been cultured, identified, and
characterized
\.isolate - ANSWERS✔-single colony isolated from a specimen and grown in
culture
\.clone - ANSWERS✔-multiple strains across locations and time that are
phenotypically and genetically identical
\.mesophiles - ANSWERS✔-moderate temperature loving microbes
\.Which phyla do taxonomists have controversy about? - ANSWERS✔-
Thaumarchaeota
\.Thaumarchaeota - ANSWERS✔-were grouped with Crenarchaeota, and their
independence on a phylogeny is not agreed upon
\.extremophiles - ANSWERS✔-lovers of extreme conditions (cold, hot, salinity)
QUESTIONS AND VERIFIED ANSWERS
\.How many domains of life are there? - ANSWERS✔-3: Bacteria; Archaea; Eukarya
\.Smallest taxonomic rank is __________. - ANSWERS✔-strain (species)
\.Largest taxonomic rank is __________. - ANSWERS✔-Domain
\.How many kingdoms are microorganisms found in? - ANSWERS✔-4
\.Genus and species are specific to _______ microorganism. - ANSWERS✔-one
\.Sequence alignment for DNA - ANSWERS✔-referenced as a percent identity,
higher the percent, higher the similarity between sequences
\.What type of genetic material do we use to identify bacteria and archaea? -
ANSWERS✔-16S rRNA
\.whole genome sequencing - ANSWERS✔-complete genetic information,
considered same species if 95% identity, can determine strains
, \.In order to extract DNA from a microbe we must _________. - ANSWERS✔-
culture the microbe
\.metagenomic sequencing - ANSWERS✔-DNA extracted from raw sample and
sequenced, identification is only as good as your database
\.strain - ANSWERS✔-an isolate that has been cultured, identified, and
characterized
\.isolate - ANSWERS✔-single colony isolated from a specimen and grown in
culture
\.clone - ANSWERS✔-multiple strains across locations and time that are
phenotypically and genetically identical
\.mesophiles - ANSWERS✔-moderate temperature loving microbes
\.Which phyla do taxonomists have controversy about? - ANSWERS✔-
Thaumarchaeota
\.Thaumarchaeota - ANSWERS✔-were grouped with Crenarchaeota, and their
independence on a phylogeny is not agreed upon
\.extremophiles - ANSWERS✔-lovers of extreme conditions (cold, hot, salinity)
