UTA MICROBIOLOGY ACTUAL EXAM PAPER
2026 QUESTIONS WITH ANSWERS GRADED
A+
◉ Z Ring Assembly. Answer: FtsZ assembles Z ring to form divisome
◉ Generation Time. Answer: time it takes to double the population
◉ Lag Phase. Answer: Cells adjust to culture medium; no change in
population
◉ What factors determine the duration of the lag phase? Answer:
Genetic makeup, media composition, initial inoculum size
◉ During which phase of the growth curve is it most appropriate for the
use of antibiotics? Answer: Log phase
◉ Following the death phase of the growth curve, ____ cells are
considered the surviving cells & are considered clinically important due
to their association with chronic infections, such as Tuberculosis.
Answer: Persisters
,◉ Log Phase. Answer: Binary fission occurs; cell replication > cell
death
◉ Stationary Phase. Answer: Resources become depleted; cell
respiration=cell death
◉ Death Phase. Answer: Endospores can form; cell replication < cell
death
◉ Close System Cultures. Answer: Have finite resources
◉ Open System Cultures. Answer: -Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
◉ Microscopic Cell Count. Answer: Cells are counted under a
microscope using a laser etched slide
◉ In using the direct microscopic cell count method, you will be able to
distinguish between live vs dead cells. Answer: False
◉ Fluorescence Staining. Answer: Cells are counted under a microscope
or flow cytometer
, ◉ In fluorescent staining, the end product results in live cells staining
___________? Answer: Green
◉ In fluorescent staining, the end product results in dead cells staining
___________? Answer: Red
◉ Coulter counting. Answer: Detects electrical resistance change due to
cell density
◉ Viable Cell Counts. Answer: Samples are diluted and grown on solid
media
◉ What type of cells are counted when using the viable plate count
method? Answer: Living
◉ Countable Range. Answer: 30-300 CFU/ml
◉ Optical Density. Answer: -Measured with spectrophotometer
-Light is passed thru culture and measured on other side
◉ Which method of measuring growth is considered an indirect
approach? Answer: Optical density
2026 QUESTIONS WITH ANSWERS GRADED
A+
◉ Z Ring Assembly. Answer: FtsZ assembles Z ring to form divisome
◉ Generation Time. Answer: time it takes to double the population
◉ Lag Phase. Answer: Cells adjust to culture medium; no change in
population
◉ What factors determine the duration of the lag phase? Answer:
Genetic makeup, media composition, initial inoculum size
◉ During which phase of the growth curve is it most appropriate for the
use of antibiotics? Answer: Log phase
◉ Following the death phase of the growth curve, ____ cells are
considered the surviving cells & are considered clinically important due
to their association with chronic infections, such as Tuberculosis.
Answer: Persisters
,◉ Log Phase. Answer: Binary fission occurs; cell replication > cell
death
◉ Stationary Phase. Answer: Resources become depleted; cell
respiration=cell death
◉ Death Phase. Answer: Endospores can form; cell replication < cell
death
◉ Close System Cultures. Answer: Have finite resources
◉ Open System Cultures. Answer: -Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
◉ Microscopic Cell Count. Answer: Cells are counted under a
microscope using a laser etched slide
◉ In using the direct microscopic cell count method, you will be able to
distinguish between live vs dead cells. Answer: False
◉ Fluorescence Staining. Answer: Cells are counted under a microscope
or flow cytometer
, ◉ In fluorescent staining, the end product results in live cells staining
___________? Answer: Green
◉ In fluorescent staining, the end product results in dead cells staining
___________? Answer: Red
◉ Coulter counting. Answer: Detects electrical resistance change due to
cell density
◉ Viable Cell Counts. Answer: Samples are diluted and grown on solid
media
◉ What type of cells are counted when using the viable plate count
method? Answer: Living
◉ Countable Range. Answer: 30-300 CFU/ml
◉ Optical Density. Answer: -Measured with spectrophotometer
-Light is passed thru culture and measured on other side
◉ Which method of measuring growth is considered an indirect
approach? Answer: Optical density
