Please check the examination details below before entering your candidate information
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Thursday 5 June 2025
Paper
Afternoon (Time: 1 hour 45 minutes)
reference 9BI0/01
Biology B
Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
Use black ink or ball-point pen.
• Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•
Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
The total mark for this paper is 90.
• The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• In question(s) marked with an asterisk (*), marks will be awarded for your ability
to structure your answer logically showing how the points that you make are
related or follow on from each other where appropriate.
PEARSON A LEVEL BIOLOGY B PAPER 1 ADVANCED BIOCHEMISTRY
COMBINED QUESTION PAPER & MARK SCHEME
Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
Turn over
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you change your mind about an D
answer, put a line through the box and then mark your new answer with a cross . O
1 Active transport is one type of cell transport mechanism. N
(a) What happens during active transport? O
(1) T
W
A ATP is hydrolysed releasing energy RI
TE
B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy IN
T
D ATP is phosphorylated using energy HI
S
(b) Which substance is transported against a concentration gradient by active AR
transport? EA
(1)
A hydrophilic molecule
B large hydrophobic molecule
C small hydrophobic molecule
D water
D
O
N
O
T
W
RI
TE
IN
T
HI
S
AR
EA
D
2
*P74459A0236*
, (c) The graph shows the change in concentration of molecule A and molecule B, as
AR they are transported into the cytoplasm of a cell.
molecule A
EA
TH
IS
IN Concentration of molecules
in the cytoplasm molecule B
W
RI
TE
T
N
20 30 0 10
O Time / mins
D Explain the differences in the changes in concentration of molecule A and
O molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)
..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
AR ..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
EA ..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
TH ..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
(Total for Question 1 = 4 marks)
IS
IN
W
RI
TE
T
N
O
D
O
3
*P74459A0336* Turn over
, 2 The diagram shows part of cellular respiration.
glucose D
O
glycolysis
N
O
molecule X
T
link reaction W
RI
and TE
Krebs cycle
carbon dioxide IN
(a) Which is molecule X? T
(1) HI
S
A glycerate 3-phosphate
AR
B glycogen EA
C lactate
D pyruvate
(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1) D
Glycolysis Link reaction Krebs cycle O
cytoplasm cytoplasm cytoplasm N
A
O
B cytoplasm mitochondria mitochondria
T
W
C mitochondria cytoplasm cytoplasm RI
TE
D mitochondria mitochondria mitochondria
IN
T
HI
S
AR
EA
4
*P74459A0436*
D
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 3 GCE
Thursday 5 June 2025
Paper
Afternoon (Time: 1 hour 45 minutes)
reference 9BI0/01
Biology B
Advanced
PAPER 1: Advanced Biochemistry, Microbiology
and Genetics
You must have: Total Marks
Scientific calculator, HB pencil, ruler
Instructions
Use black ink or ball-point pen.
• Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•
Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
The total mark for this paper is 90.
• The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• In question(s) marked with an asterisk (*), marks will be awarded for your ability
to structure your answer logically showing how the points that you make are
related or follow on from each other where appropriate.
PEARSON A LEVEL BIOLOGY B PAPER 1 ADVANCED BIOCHEMISTRY
COMBINED QUESTION PAPER & MARK SCHEME
Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
Turn over
, Answer ALL questions.
Some questions must be answered with a cross in a box . If you change your mind about an D
answer, put a line through the box and then mark your new answer with a cross . O
1 Active transport is one type of cell transport mechanism. N
(a) What happens during active transport? O
(1) T
W
A ATP is hydrolysed releasing energy RI
TE
B ATP is hydrolysed using energy
C ATP is phosphorylated releasing energy IN
T
D ATP is phosphorylated using energy HI
S
(b) Which substance is transported against a concentration gradient by active AR
transport? EA
(1)
A hydrophilic molecule
B large hydrophobic molecule
C small hydrophobic molecule
D water
D
O
N
O
T
W
RI
TE
IN
T
HI
S
AR
EA
D
2
*P74459A0236*
, (c) The graph shows the change in concentration of molecule A and molecule B, as
AR they are transported into the cytoplasm of a cell.
molecule A
EA
TH
IS
IN Concentration of molecules
in the cytoplasm molecule B
W
RI
TE
T
N
20 30 0 10
O Time / mins
D Explain the differences in the changes in concentration of molecule A and
O molecule B between 0 and 10 minutes and between 20 and 30 minutes.
(2)
..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
AR ..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
EA ..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
TH ..... .. .. .... .... .... .... ........ .... .... ... ........ .... .... ........ .... .... .... .... .... .... ........ .... .... ... ........ .... .. .. .... ........ .... .... .... .... ........ .... .... .... ....... .... .. .. .... ............ .... .... .... .... ........ .... .... .... ....... ... . .... .... .... ... ..... .. .
(Total for Question 1 = 4 marks)
IS
IN
W
RI
TE
T
N
O
D
O
3
*P74459A0336* Turn over
, 2 The diagram shows part of cellular respiration.
glucose D
O
glycolysis
N
O
molecule X
T
link reaction W
RI
and TE
Krebs cycle
carbon dioxide IN
(a) Which is molecule X? T
(1) HI
S
A glycerate 3-phosphate
AR
B glycogen EA
C lactate
D pyruvate
(b) Which row of the table shows the locations of glycolysis, the link reaction and the
Krebs cycle in eukaryotic cells?
(1) D
Glycolysis Link reaction Krebs cycle O
cytoplasm cytoplasm cytoplasm N
A
O
B cytoplasm mitochondria mitochondria
T
W
C mitochondria cytoplasm cytoplasm RI
TE
D mitochondria mitochondria mitochondria
IN
T
HI
S
AR
EA
4
*P74459A0436*
D
