Stage opdracht 951 snuffelen aan onderzoek
Report 951 Research of infections with sodium hyper chloride
,Index
Abbreviations.........................................................................................................................................1
Abstract..................................................................................................................................................2
1.0 Review of the Literature...................................................................................................................3
1.1 Introduction..................................................................................................................................3
1.2 Production of Hortensia................................................................................................................3
1.3 History of the Hortensia...............................................................................................................4
1.4 tissue culture................................................................................................................................4
1.4.1 plant tissue culture................................................................................................................4
1.4.2 History (plant) tissue culture..................................................................................................5
1.5 infections......................................................................................................................................5
2 Materials and Methods.......................................................................................................................6
2.1 Collection and Management of Cultures......................................................................................6
2.1.1 Plant Material........................................................................................................................6
2.1.2 Disinfection treatments.........................................................................................................6
2.1.3 Culture Media and their Preparation.....................................................................................7
3.0 results...............................................................................................................................................9
3.1 Contamination rates of the three varieties depending on procedure of disinfection...................9
3.1.1 table infections week 1 Royal Red.........................................................................................9
3.1.2 table infections week 2 Royal Red.......................................................................................10
3.1.3 table infections week 3 Royal Red.......................................................................................10
3.1.4 table infections week 4 Royal Red.......................................................................................11
3.1.5 table infections week 1 hydrangea Quercifolia....................................................................12
3.1.6 table infections week 2 hydrangea Quercifolia....................................................................13
3.1.7 table infections week 3 hydrangea Quercifolia....................................................................14
3.1.9 table infections week 1 Bobo...............................................................................................16
3.1.10 table infections week 2 Bobo.............................................................................................17
3.1.11 table infections week 3 Bobo.............................................................................................17
3.1.12 table infections week 4 Bobo.............................................................................................18
3.1.13 graphics of the variety.......................................................................................................19
3.2 Effect of recovery of explants on three different media.............................................................21
3.3 relationship between the three media.......................................................................................21
4.0 Discussion and future outlook........................................................................................................23
5.0 List of reference..............................................................................................................................25
, Abbreviations
Ms Murashige & Skoog medium
PGR Plant Growth Regulator
NaClO Sodium hypochlorite
C2H5OH ethanol
IAA indole-3-acetic acid (auxin - plant hormone)
1
Report 951 Research of infections with sodium hyper chloride
,Index
Abbreviations.........................................................................................................................................1
Abstract..................................................................................................................................................2
1.0 Review of the Literature...................................................................................................................3
1.1 Introduction..................................................................................................................................3
1.2 Production of Hortensia................................................................................................................3
1.3 History of the Hortensia...............................................................................................................4
1.4 tissue culture................................................................................................................................4
1.4.1 plant tissue culture................................................................................................................4
1.4.2 History (plant) tissue culture..................................................................................................5
1.5 infections......................................................................................................................................5
2 Materials and Methods.......................................................................................................................6
2.1 Collection and Management of Cultures......................................................................................6
2.1.1 Plant Material........................................................................................................................6
2.1.2 Disinfection treatments.........................................................................................................6
2.1.3 Culture Media and their Preparation.....................................................................................7
3.0 results...............................................................................................................................................9
3.1 Contamination rates of the three varieties depending on procedure of disinfection...................9
3.1.1 table infections week 1 Royal Red.........................................................................................9
3.1.2 table infections week 2 Royal Red.......................................................................................10
3.1.3 table infections week 3 Royal Red.......................................................................................10
3.1.4 table infections week 4 Royal Red.......................................................................................11
3.1.5 table infections week 1 hydrangea Quercifolia....................................................................12
3.1.6 table infections week 2 hydrangea Quercifolia....................................................................13
3.1.7 table infections week 3 hydrangea Quercifolia....................................................................14
3.1.9 table infections week 1 Bobo...............................................................................................16
3.1.10 table infections week 2 Bobo.............................................................................................17
3.1.11 table infections week 3 Bobo.............................................................................................17
3.1.12 table infections week 4 Bobo.............................................................................................18
3.1.13 graphics of the variety.......................................................................................................19
3.2 Effect of recovery of explants on three different media.............................................................21
3.3 relationship between the three media.......................................................................................21
4.0 Discussion and future outlook........................................................................................................23
5.0 List of reference..............................................................................................................................25
, Abbreviations
Ms Murashige & Skoog medium
PGR Plant Growth Regulator
NaClO Sodium hypochlorite
C2H5OH ethanol
IAA indole-3-acetic acid (auxin - plant hormone)
1
