TEST BANK FOR
Becker's World of the
Cell by Jeff Hardin
10th Edition
1
, TABLE OF CONTENT
• Chapter 1: Using Immunofluorescence to Identify Specific Cell Components
• Chapter 2: Determining the Chemical Fingerprint of a Cell Using Mass Spectrometry
• Chapter 3: Using X-ray Crystallography to Determine Protein Structure
• Chapter 4: Using Centrifugation to Isolate Organelles
• Chapter 5: Measuring How Molecules Bind One Another Using Isothermal Titration Calorimetry
• Chapter 6: Determining Km and Vmax Using Enzyme Assays
• Chapter 7: SDS—Polyacrylamide Gel Electrophoresis (SDS—PAGE) of Membrane Proteins
• Chapter 8: Expression of Heterologous Membrane Proteins in Frog Oocytes
• Chapter 9: Using Isotopic Labeling to Determine the Fate of Atoms in a Metabolic Pathway
• Chapter 10: Visualizing Cellular Structures with Three-Dimensional Electron Microscopy
• Chapter 11: Determining Absorption and Action Spectra via Spectrophotometry
• Chapter 12: Visualizing Vesicles at the Cell Surface Using Total Internal Reflection Microscopy
(TIRF)
• Chapter 13: Studying the Dynamic Cytoskeleton
• Chapter 14: Watching Motors Too Small to See
• Chapter 15: Building an ECM from Scratch
• Chapter 16: FISHing for Specific Sequences
• Chapter 17: The Polymerase Chain Reaction
• Chapter 18: Hunting for DNA-Protein Interactions
• Chapter 19: Protein Localization Using Fluorescent Fusion Proteins
• Chapter 20: Gene Knockdown via RNAi
• Chapter 21: DNA Cloning
• Chapter 22: Patch Clamping
• Chapter 23: Calcium Indicators and Ionophores
• Chapter 24: Measuring Cells Millions at a Time (FACS -Analysis)
• Chapter 25: Using Mendel's Rules to Predict Human Disease
• Chapter 26: "Rational" Drugs in the Fight
2
,Against Cancer Human Connections
• Chapter 1: The Immortal Cells of Henrietta Lacks (The origin of the first human cultured cell line)
• Chapter 2: Taking a Deeper Look -- Magnetic Resonance Imaging (MRI) (How an MRI can image
human tissues)
3
, • Chapter 3: Aggregated Proteins and Alzheimer Disease (Protein misfolding and human disease)
• Chapter 4: When Cellular "Breakdown" Breaks Down (Diseases caused by lysosome dysfunction)
• Chapter 5: The "Potential" of Food to Provide Energy (Calorie contents of foods)
• Chapter 6: ACE Inhibitors: Enzyme Activity as the Difference Between Life and Death (Blood
pressure medication based on snake venom)
• Chapter 8: Membrane Transport, Cystic Fibrosis, and the Prospects for Gene Therapy
• Chapter 9: What Happens to the Sugar? (Dietary food intake and metabolism)
• Chapter 10: A Diet Worth Dying For? (The dangers of using uncouplers such as DNP to lose
weight)
• Chapter 11: How Do Plants Put on Sunscreen? (How the plant xanthophyll cycle dissipates excess
solar energy)
• Chapter 12: It's All in the Family (LDL receptors, cholesterol uptake, hypercholesterolemia)
• Chapter 13: When Actin Kills (Listeria infection and actin polymerization)
• Chapter 14: Dyneins Help Us Tell Left from Right (Motors and left-right axis specification)
• Chapter 15: The Costly Effects of Weak Adhesion (Blistering diseases of the skin)
• Chapter 16: Lamins and Premature Aging (Progeria and the nuclear envelope)
• Chapter 17: Children of the Moon (Xeroderma pigmentosum and DNA repair)
• Chapter 19: To Catch a Killer: The Problem of Antibiotic Resistance in Bacteria
• Chapter 20: The Epigenome: Methylation and Disease (Rett syndrome and imprinting disorders)
• Chapter 21: More Than Your Fingertips: Identifying Genetic "Fingerprints" (STRs in genetic
identification)
• Chapter 22: In the Search for the Fountain of Youth, Are People Paying for Poison? (Cosmetic uses
of Botulinum toxin)
• Chapter 23: How to Prevent a Heart Attack (Nitric oxide -signaling and vasoconstriction)
• Chapter 24: What do Ethnobotany and Cancer Have in Common? (Taxol and cancer treatment)
• Chapter 25: When Meiosis Goes Awry (Down syndrome and nondisjunction)
• Chapter 26: Molecular Sleuthing in Cancer Diagnosis (Molecular analysis of breast cancer)
4
Becker's World of the
Cell by Jeff Hardin
10th Edition
1
, TABLE OF CONTENT
• Chapter 1: Using Immunofluorescence to Identify Specific Cell Components
• Chapter 2: Determining the Chemical Fingerprint of a Cell Using Mass Spectrometry
• Chapter 3: Using X-ray Crystallography to Determine Protein Structure
• Chapter 4: Using Centrifugation to Isolate Organelles
• Chapter 5: Measuring How Molecules Bind One Another Using Isothermal Titration Calorimetry
• Chapter 6: Determining Km and Vmax Using Enzyme Assays
• Chapter 7: SDS—Polyacrylamide Gel Electrophoresis (SDS—PAGE) of Membrane Proteins
• Chapter 8: Expression of Heterologous Membrane Proteins in Frog Oocytes
• Chapter 9: Using Isotopic Labeling to Determine the Fate of Atoms in a Metabolic Pathway
• Chapter 10: Visualizing Cellular Structures with Three-Dimensional Electron Microscopy
• Chapter 11: Determining Absorption and Action Spectra via Spectrophotometry
• Chapter 12: Visualizing Vesicles at the Cell Surface Using Total Internal Reflection Microscopy
(TIRF)
• Chapter 13: Studying the Dynamic Cytoskeleton
• Chapter 14: Watching Motors Too Small to See
• Chapter 15: Building an ECM from Scratch
• Chapter 16: FISHing for Specific Sequences
• Chapter 17: The Polymerase Chain Reaction
• Chapter 18: Hunting for DNA-Protein Interactions
• Chapter 19: Protein Localization Using Fluorescent Fusion Proteins
• Chapter 20: Gene Knockdown via RNAi
• Chapter 21: DNA Cloning
• Chapter 22: Patch Clamping
• Chapter 23: Calcium Indicators and Ionophores
• Chapter 24: Measuring Cells Millions at a Time (FACS -Analysis)
• Chapter 25: Using Mendel's Rules to Predict Human Disease
• Chapter 26: "Rational" Drugs in the Fight
2
,Against Cancer Human Connections
• Chapter 1: The Immortal Cells of Henrietta Lacks (The origin of the first human cultured cell line)
• Chapter 2: Taking a Deeper Look -- Magnetic Resonance Imaging (MRI) (How an MRI can image
human tissues)
3
, • Chapter 3: Aggregated Proteins and Alzheimer Disease (Protein misfolding and human disease)
• Chapter 4: When Cellular "Breakdown" Breaks Down (Diseases caused by lysosome dysfunction)
• Chapter 5: The "Potential" of Food to Provide Energy (Calorie contents of foods)
• Chapter 6: ACE Inhibitors: Enzyme Activity as the Difference Between Life and Death (Blood
pressure medication based on snake venom)
• Chapter 8: Membrane Transport, Cystic Fibrosis, and the Prospects for Gene Therapy
• Chapter 9: What Happens to the Sugar? (Dietary food intake and metabolism)
• Chapter 10: A Diet Worth Dying For? (The dangers of using uncouplers such as DNP to lose
weight)
• Chapter 11: How Do Plants Put on Sunscreen? (How the plant xanthophyll cycle dissipates excess
solar energy)
• Chapter 12: It's All in the Family (LDL receptors, cholesterol uptake, hypercholesterolemia)
• Chapter 13: When Actin Kills (Listeria infection and actin polymerization)
• Chapter 14: Dyneins Help Us Tell Left from Right (Motors and left-right axis specification)
• Chapter 15: The Costly Effects of Weak Adhesion (Blistering diseases of the skin)
• Chapter 16: Lamins and Premature Aging (Progeria and the nuclear envelope)
• Chapter 17: Children of the Moon (Xeroderma pigmentosum and DNA repair)
• Chapter 19: To Catch a Killer: The Problem of Antibiotic Resistance in Bacteria
• Chapter 20: The Epigenome: Methylation and Disease (Rett syndrome and imprinting disorders)
• Chapter 21: More Than Your Fingertips: Identifying Genetic "Fingerprints" (STRs in genetic
identification)
• Chapter 22: In the Search for the Fountain of Youth, Are People Paying for Poison? (Cosmetic uses
of Botulinum toxin)
• Chapter 23: How to Prevent a Heart Attack (Nitric oxide -signaling and vasoconstriction)
• Chapter 24: What do Ethnobotany and Cancer Have in Common? (Taxol and cancer treatment)
• Chapter 25: When Meiosis Goes Awry (Down syndrome and nondisjunction)
• Chapter 26: Molecular Sleuthing in Cancer Diagnosis (Molecular analysis of breast cancer)
4