BIOD 171 MICROBIOLOGY LAB 1 LAB 9 STUDY
GUIDE 2026 QUESTIONS WITH COMPLETE
SOLUTIONS GRADED A+
◉ Negative Stain. Answer:
◉ Acid-Fast Stain. Answer:
◉ Capsule Stain. Answer:
◉ Endospore Stain. Answer:
◉ Wet Mount and Hanging Drop. Answer: allow observation of living
cells to determine motility
◉ Flagella Stain. Answer: (prepared stain) allows for observation of
flagella
◉ Columbia CNA with 5% Sheep Blood Agar. Answer:
differential/selective medium that allows for growth of Gram-
positive and inhibit growth of Gram-negative organisms
,◉ Know the Microscopes Parts!. Answer:
◉ Base. Answer: supports the microscope
◉ Mechanical Stage. Answer:
◉ Condenser. Answer: focuses light through the specimen
◉ Arm. Answer: along with the base, used to support the
microscope when carried
◉ Objective Lens. Answer: have magnification ranging from 4x to
40x
◉ Light Source/Illuminator. Answer: projects light upward through
the diaphragm and stage
◉ Diaphragm. Answer: regulates the amount of light on the
specimen
◉ Ocular Lens. Answer: has a magnification of 10x.; aka eyepiece
◉ Revolving Nosepiece. Answer: rotates the objective lenses
, ◉ Stage Clips. Answer: holds the slide in place
◉ Slide. Answer: where the specimen is placed
◉ Specimen. Answer: the object being studied
◉ Coarse Adjustment Knob. Answer: moves the stage up and down
for focusing
◉ Fine Adjustment Knob. Answer: moves the stage slightly to
sharpen the image
◉ Know the proper procedure for focusing the microscope:. Answer:
1. On the low-power objective, use the coarse objective only
2. When higher power objectives are used, the fine adjustment knob
can be changed to bring the object into focus.
◉ Know the proper procedure when viewing a slide:. Answer: 1.
turn on microscope
2. Choose slide
3. Put it on stage
4. Lock the slide in place
GUIDE 2026 QUESTIONS WITH COMPLETE
SOLUTIONS GRADED A+
◉ Negative Stain. Answer:
◉ Acid-Fast Stain. Answer:
◉ Capsule Stain. Answer:
◉ Endospore Stain. Answer:
◉ Wet Mount and Hanging Drop. Answer: allow observation of living
cells to determine motility
◉ Flagella Stain. Answer: (prepared stain) allows for observation of
flagella
◉ Columbia CNA with 5% Sheep Blood Agar. Answer:
differential/selective medium that allows for growth of Gram-
positive and inhibit growth of Gram-negative organisms
,◉ Know the Microscopes Parts!. Answer:
◉ Base. Answer: supports the microscope
◉ Mechanical Stage. Answer:
◉ Condenser. Answer: focuses light through the specimen
◉ Arm. Answer: along with the base, used to support the
microscope when carried
◉ Objective Lens. Answer: have magnification ranging from 4x to
40x
◉ Light Source/Illuminator. Answer: projects light upward through
the diaphragm and stage
◉ Diaphragm. Answer: regulates the amount of light on the
specimen
◉ Ocular Lens. Answer: has a magnification of 10x.; aka eyepiece
◉ Revolving Nosepiece. Answer: rotates the objective lenses
, ◉ Stage Clips. Answer: holds the slide in place
◉ Slide. Answer: where the specimen is placed
◉ Specimen. Answer: the object being studied
◉ Coarse Adjustment Knob. Answer: moves the stage up and down
for focusing
◉ Fine Adjustment Knob. Answer: moves the stage slightly to
sharpen the image
◉ Know the proper procedure for focusing the microscope:. Answer:
1. On the low-power objective, use the coarse objective only
2. When higher power objectives are used, the fine adjustment knob
can be changed to bring the object into focus.
◉ Know the proper procedure when viewing a slide:. Answer: 1.
turn on microscope
2. Choose slide
3. Put it on stage
4. Lock the slide in place
