(MB) ASCP Practice Exam | (Latest
2026/2027 Update) 160 Questions &
Answers | Grade A | 100% Correct
Description
This MB (ASCP) Technologist in Molecular Biology
study guide is a 160 Q&A, it prepares candidates
for the certification exam by focusing on molecular
techniques, nucleic acid isolation, and clinical
applications. These guides typically feature
structured content covering molecular diagnostics,
laboratory operations, and detailed, exam-aligned
practice questions.
Key Components of this MB (ASCP) Study
Guide
Targeted Content Coverage: Reviews essential
topics including nucleic acid extraction,
amplification (PCR), sequencing, molecular
microbiology, genetics, and oncology.
Practice Questions & Answers: Includes multiple-
choice questions similar to the exam, with detailed
answers to enhance retention.
1
, Quiz_________________?
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base -
Answer☑️
Anti-codon
Quiz_________________?
According to Chargaff's rule of base pairing, adenine pairs with: -
Answer☑️
Thymine
Quiz_________________?
What genes would be screened in a breast cancer panel? -
Answer☑️
HER2, ERBB2, BRCA1
2
, Quiz_________________?
Next Generation Sequencing uses: -
Answer☑️
Short sequence reads
Quiz_________________?
Purines and pyrimidines differ from each other in that: -
Answer☑️
Purines have two rings; pyrimidines have one ring
Quiz_________________?
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine -
Answer☑️
Adenine and guanine
Quiz_________________?
3
, What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs -
Answer☑️
1 in 1,000,000,000 base pairs
Quiz_________________?
The rate of DNA migration through an agarose gel during
electrophoresis does not depend on which of the following
factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule -
Answer☑️
Nucleotide sequence of the molecule
Quiz_________________?
What are the phases in a qPCR Amplification Plot?
4
2026/2027 Update) 160 Questions &
Answers | Grade A | 100% Correct
Description
This MB (ASCP) Technologist in Molecular Biology
study guide is a 160 Q&A, it prepares candidates
for the certification exam by focusing on molecular
techniques, nucleic acid isolation, and clinical
applications. These guides typically feature
structured content covering molecular diagnostics,
laboratory operations, and detailed, exam-aligned
practice questions.
Key Components of this MB (ASCP) Study
Guide
Targeted Content Coverage: Reviews essential
topics including nucleic acid extraction,
amplification (PCR), sequencing, molecular
microbiology, genetics, and oncology.
Practice Questions & Answers: Includes multiple-
choice questions similar to the exam, with detailed
answers to enhance retention.
1
, Quiz_________________?
Which of the following is not a component of a nucleotide?
Phosphate group
Anti-codon
Ribose sugar
Nitrogen base -
Answer☑️
Anti-codon
Quiz_________________?
According to Chargaff's rule of base pairing, adenine pairs with: -
Answer☑️
Thymine
Quiz_________________?
What genes would be screened in a breast cancer panel? -
Answer☑️
HER2, ERBB2, BRCA1
2
, Quiz_________________?
Next Generation Sequencing uses: -
Answer☑️
Short sequence reads
Quiz_________________?
Purines and pyrimidines differ from each other in that: -
Answer☑️
Purines have two rings; pyrimidines have one ring
Quiz_________________?
The purines are:
Cytosine and uracil
Adenine and thymine
Thymine and cytosine
Adenine and guanine -
Answer☑️
Adenine and guanine
Quiz_________________?
3
, What is the rate of mutation per round of DNA replication?
1 in 1,000 base pairs
1 in 10,000 base pairs
1 in 1,000,000 base pairs
1 in 1,000,000,000 base pairs -
Answer☑️
1 in 1,000,000,000 base pairs
Quiz_________________?
The rate of DNA migration through an agarose gel during
electrophoresis does not depend on which of the following
factors?
Net charge of the molecule
Size of the molecule
Shape of the molecule
Nucleotide sequence of the molecule -
Answer☑️
Nucleotide sequence of the molecule
Quiz_________________?
What are the phases in a qPCR Amplification Plot?
4
