MLT ASCP EVALUATION EXAM 2026 QUESTIONS AND
ANSWERS RATED A+
✔✔A;
Fusarium species is the most likely associated with mycotic keratitis.
Trichophyton rubrum is a dermatophyte that commonly causes an itching, scaling skin
infection of the feet, known as tinea pedis. Scedosporium apiospermum is commonly
associated with sinusitis. Aspergillus niger typically causes otitis externa and can also
be associated with sinusitis. - ✔✔Which of the following species or organisms is the
most likely to be the cause of mycotic keratitis (fungal eye infection)?
A. Fusarium species
B. Trichophyton rubrum.
C. Scedosporium apiospermum
D. Aspergillus niger
✔✔A;
Oxalate, EDTA, and citrate are anticoagulants that inhibit clot formation. - ✔✔Which of
the following blood additives is most useful for serum collection:
A. Polymer barrier
B. Oxalate
C. EDTA
D. Citrate
✔✔B;
This patient is most likely suffering from an immediate-acting coagulation inhibitor; most
commonly, lupus anticoagulant. Notice that the addition of normal pooled plasma does
not correct upon initial or incubated mix, which means that the inhibitor is not time or
temperature-dependent.
Factor VIII is not the correct answer as a factor deficiency would have corrected upon
the addition of normal pooled plasma. Factor VII is not the correct answer, as the aPTT
assay does not account for factor VII activity or concentration. - ✔✔The laboratorian
completed the mixing study ordered for John Doe. The results are as follows:
Initial aPTT result: 167 seconds
Initial 1:1 Mix with Normal Pooled Plasma: 158 seconds
Incubated 1:1 Mix with Normal Pooled Plasma: 150 seconds
Which of the choices below would most likely explain the results for this patient?
A. Factor VIII deficiency
B. Immediate-acting coagulation inhibitor
,C. Time/temperature-dependent coagulation inhibitor
D. Factor VII deficiency
✔✔A;
HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found
on the actual surface of the virus. HbeAg is present in the blood when the hepatitis B
viruses are replicating, indicating an active infection. Anti-Hbc IgM is present due to the
immune response to the presence of the hepatitis core antigen and indicates an acute
infection. Anti-HBs is generally interpreted as indicating recovery and immunity from
hepatitis B virus infection, according to the CDC. - ✔✔Given the following results, what
is the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative
A. acute infection
B. chronic infection
C. immunization
D. susceptible
✔✔A;
Albumin is a "negative" acute phase protein since it is found in decreased levels during
acute phase response. Alpha-1-antitrypsin, fibrinogen, and ceruloplasmin are all
"positive" acute phase proteins that are found in increased levels during acute phase
response. - ✔✔Which one of the following usually shows a decrease during an acute
phase response?
A. Albumin
B. Alpha-1 Antitrypsin
C. Fibrinogen
D. Ceruloplasmin
✔✔False
Because lower titers could be due to both passive and immune anti-D, in the absence of
results that suggest immune anti-D, routine antibody titration is not a good use of time
compared to assuming that anti-D is passive.
Best practice guidelines do NOT recommend routine titration for women known to be
injected with RhIg and exhibiting a 2+ or less reaction with D+ red cells consistent with
passive anti-D from RhIg. - ✔✔True/False
A pregnant female who received RhIg at 28 weeks gestation has a positive antibody
screen at delivery. If the antibody has been confirmed as anti-D alone and reacts 1+ in
the indirect antiglobulin test with D+ red cells, performing a titration to investigate if the
anti-D is immune is good practice.
, ✔✔1. B
2. D
3. A
4. C
Red to Brown Urine: porphobilinogen, hematuria, myoglobinuria, etc.
Green: Food colorings; Increased carotene in the diet;
Pseudomonas aeruginosa infection
Yellow: bilirubin, bile pigments
White: phosphates, other crytals - ✔✔Match urine color with substance that might have
been responsible:
1. Phosphates
2. Bilirubin
3. Pseudomonas
4. Porphobilinogen
A. Blue to green
B. White
C. Red to brown
D. Yellow
✔✔A;
The production of long, tapered phialides is one of the key identifying features of
Trichoderma species. In contrast, Penicillium species, produce phialides with blunt
ends.
The phialides of Beauveria species are geniculate, forming a zig-zag pattern. - ✔✔The
arrows in the image on the right point to single, long, tapered phialides that extend
laterally from either side of the hyphae. This is an identifying feature of which of the
fungi listed below?
A. Trichoderma species
B. Penicillium species
C. Beauveria species
✔✔A;
The DAT is most likely to be negative in ABO HDFN. It's possible that the washing done
as part of the DAT may break the bonds between anti-A (or anti-B) and the newborn's
poorly developed A (or B) antigens. - ✔✔For which of the following antibodies is the
DAT most likely to be negative when testing a newborn for possible HDFN?
A. anti-A
B. anti-c
C. anti-D
ANSWERS RATED A+
✔✔A;
Fusarium species is the most likely associated with mycotic keratitis.
Trichophyton rubrum is a dermatophyte that commonly causes an itching, scaling skin
infection of the feet, known as tinea pedis. Scedosporium apiospermum is commonly
associated with sinusitis. Aspergillus niger typically causes otitis externa and can also
be associated with sinusitis. - ✔✔Which of the following species or organisms is the
most likely to be the cause of mycotic keratitis (fungal eye infection)?
A. Fusarium species
B. Trichophyton rubrum.
C. Scedosporium apiospermum
D. Aspergillus niger
✔✔A;
Oxalate, EDTA, and citrate are anticoagulants that inhibit clot formation. - ✔✔Which of
the following blood additives is most useful for serum collection:
A. Polymer barrier
B. Oxalate
C. EDTA
D. Citrate
✔✔B;
This patient is most likely suffering from an immediate-acting coagulation inhibitor; most
commonly, lupus anticoagulant. Notice that the addition of normal pooled plasma does
not correct upon initial or incubated mix, which means that the inhibitor is not time or
temperature-dependent.
Factor VIII is not the correct answer as a factor deficiency would have corrected upon
the addition of normal pooled plasma. Factor VII is not the correct answer, as the aPTT
assay does not account for factor VII activity or concentration. - ✔✔The laboratorian
completed the mixing study ordered for John Doe. The results are as follows:
Initial aPTT result: 167 seconds
Initial 1:1 Mix with Normal Pooled Plasma: 158 seconds
Incubated 1:1 Mix with Normal Pooled Plasma: 150 seconds
Which of the choices below would most likely explain the results for this patient?
A. Factor VIII deficiency
B. Immediate-acting coagulation inhibitor
,C. Time/temperature-dependent coagulation inhibitor
D. Factor VII deficiency
✔✔A;
HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found
on the actual surface of the virus. HbeAg is present in the blood when the hepatitis B
viruses are replicating, indicating an active infection. Anti-Hbc IgM is present due to the
immune response to the presence of the hepatitis core antigen and indicates an acute
infection. Anti-HBs is generally interpreted as indicating recovery and immunity from
hepatitis B virus infection, according to the CDC. - ✔✔Given the following results, what
is the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative
A. acute infection
B. chronic infection
C. immunization
D. susceptible
✔✔A;
Albumin is a "negative" acute phase protein since it is found in decreased levels during
acute phase response. Alpha-1-antitrypsin, fibrinogen, and ceruloplasmin are all
"positive" acute phase proteins that are found in increased levels during acute phase
response. - ✔✔Which one of the following usually shows a decrease during an acute
phase response?
A. Albumin
B. Alpha-1 Antitrypsin
C. Fibrinogen
D. Ceruloplasmin
✔✔False
Because lower titers could be due to both passive and immune anti-D, in the absence of
results that suggest immune anti-D, routine antibody titration is not a good use of time
compared to assuming that anti-D is passive.
Best practice guidelines do NOT recommend routine titration for women known to be
injected with RhIg and exhibiting a 2+ or less reaction with D+ red cells consistent with
passive anti-D from RhIg. - ✔✔True/False
A pregnant female who received RhIg at 28 weeks gestation has a positive antibody
screen at delivery. If the antibody has been confirmed as anti-D alone and reacts 1+ in
the indirect antiglobulin test with D+ red cells, performing a titration to investigate if the
anti-D is immune is good practice.
, ✔✔1. B
2. D
3. A
4. C
Red to Brown Urine: porphobilinogen, hematuria, myoglobinuria, etc.
Green: Food colorings; Increased carotene in the diet;
Pseudomonas aeruginosa infection
Yellow: bilirubin, bile pigments
White: phosphates, other crytals - ✔✔Match urine color with substance that might have
been responsible:
1. Phosphates
2. Bilirubin
3. Pseudomonas
4. Porphobilinogen
A. Blue to green
B. White
C. Red to brown
D. Yellow
✔✔A;
The production of long, tapered phialides is one of the key identifying features of
Trichoderma species. In contrast, Penicillium species, produce phialides with blunt
ends.
The phialides of Beauveria species are geniculate, forming a zig-zag pattern. - ✔✔The
arrows in the image on the right point to single, long, tapered phialides that extend
laterally from either side of the hyphae. This is an identifying feature of which of the
fungi listed below?
A. Trichoderma species
B. Penicillium species
C. Beauveria species
✔✔A;
The DAT is most likely to be negative in ABO HDFN. It's possible that the washing done
as part of the DAT may break the bonds between anti-A (or anti-B) and the newborn's
poorly developed A (or B) antigens. - ✔✔For which of the following antibodies is the
DAT most likely to be negative when testing a newborn for possible HDFN?
A. anti-A
B. anti-c
C. anti-D
