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Microbiology Lab Midterm Review
2026 Questions and Answers (100%
Correct Answers) Already Graded A+
What is microbiology? Ans: The study of microbes that are
extremely small (microscopic) living organisms and certain
non-living entities.
© 2026 Assignment
Why study microbiology? Ans: we study microbiology because
microorganisms play significant roles in our lives, are
essential for life on this plant, produce oxygen in our
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atmosphere and are involved in the decomposition of dead
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organisms and waste.
While microbiology can be an interesting and exciting
experience, what should you be aware of? Ans: biosafety levels
for infectious agents.
What will be dangerous in the Lab and can result in injury or
infection? Ans: eating, drinking, smoking
What is the meaning of BSL? Ans: Biosafety levels-provides a
set of minimum standards for laboratory practices, facilities,
and equipment to be used hen handling organisms at each
level.
Organisms that present a minimal threat to the environment
and lab personnel, and do not typically cause disease in
healthy individuals, are in what biosafety level? Ans: BSL1 ex.
subtitis, Escherichia coli (most stains), Rhodospirillum rib, and
lactobacillus acidophilus
Organisms that present a moderate environmental/health
hazard are in what biosafety level? Ans: BSL 2 ex. Salmonella,
, 2
staphylococcus, aureus, clostridium dificile and borrelia
burgdorferi.
Organisms that are associated with respiratory transmission
and serious or lethal diseases (local or exotic origin), usually
personnel handles microbes in class I or II (BSC) are in what
biosafety level? Ans: BSL3 ex. Bacillus anthacis,
mycobacterium tuberculosis and west nile virus
Organisms that have a great potential for lethal infection, rigid
control of ventilation and waste management, usually
© 2026 Assignment
personnel handles microbes in class III (BSC), are in what
biosafety level? Ans: BSL 4 ex. ebola, marburg and lassa fever
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viruses
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What is the meaning of BSC? Ans: Biosafety cabinets
Biosafety cabinets are classified into Ans: three categories, all
of which draw air into the cabinet to minimize microbial
contamination back into the room.
What is the meaning of PPE? Name some examples. Ans:
Personal Protective Equipement
ex. laboratory coats, and gloves, eye, face protection
What component that is always present in experimental? Ans:
control or controls
What do microbiological experimentation tests determine?
Ans: The ability of an organism to use or produce some
chemical, or to determine the presence or absence of a specific
organism in a sample.
, 3
What does a positive result in the test indicate? Ans: The
microbe has the ability or is present in the sample.
What does a negative result in the test indicate? Ans: A lack of
that ability or absence in the sample
Tests should give a _________for specimens that are positive,
and a _________ for specimens that are negative. Ans: Positive;
negative
© 2026 Assignment
False positive is attributed to Ans: inadequate specificity
False negative is attributed to Ans: inadequate sensitivity
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Expert
Inadequate specificity Ans: an inability to discriminate
between the chemical or organism in a question and similar
chemicals or organisms would yield false-positive results
which are similar chemicals or organism was being tested.
Inadequate sensitivity Ans: an inability to detect small
amounts of the chemical or organism in question would yield a
false negative result.
Write the formulas for Sensitivity Ans: True positives/True
positive + false negatives
Write the formulas for specificity Ans: True negatives/true
negatives + false positives
Biology is Ans: the study of living organism
Microbiology is Ans: an advanced biology course
Living microbes are known as Ans: cellular microbes or
microorganisms
Microbiology Lab Midterm Review
2026 Questions and Answers (100%
Correct Answers) Already Graded A+
What is microbiology? Ans: The study of microbes that are
extremely small (microscopic) living organisms and certain
non-living entities.
© 2026 Assignment
Why study microbiology? Ans: we study microbiology because
microorganisms play significant roles in our lives, are
essential for life on this plant, produce oxygen in our
Guru01 - Stuvia
atmosphere and are involved in the decomposition of dead
Expert
organisms and waste.
While microbiology can be an interesting and exciting
experience, what should you be aware of? Ans: biosafety levels
for infectious agents.
What will be dangerous in the Lab and can result in injury or
infection? Ans: eating, drinking, smoking
What is the meaning of BSL? Ans: Biosafety levels-provides a
set of minimum standards for laboratory practices, facilities,
and equipment to be used hen handling organisms at each
level.
Organisms that present a minimal threat to the environment
and lab personnel, and do not typically cause disease in
healthy individuals, are in what biosafety level? Ans: BSL1 ex.
subtitis, Escherichia coli (most stains), Rhodospirillum rib, and
lactobacillus acidophilus
Organisms that present a moderate environmental/health
hazard are in what biosafety level? Ans: BSL 2 ex. Salmonella,
, 2
staphylococcus, aureus, clostridium dificile and borrelia
burgdorferi.
Organisms that are associated with respiratory transmission
and serious or lethal diseases (local or exotic origin), usually
personnel handles microbes in class I or II (BSC) are in what
biosafety level? Ans: BSL3 ex. Bacillus anthacis,
mycobacterium tuberculosis and west nile virus
Organisms that have a great potential for lethal infection, rigid
control of ventilation and waste management, usually
© 2026 Assignment
personnel handles microbes in class III (BSC), are in what
biosafety level? Ans: BSL 4 ex. ebola, marburg and lassa fever
Guru01 - Stuvia
viruses
Expert
What is the meaning of BSC? Ans: Biosafety cabinets
Biosafety cabinets are classified into Ans: three categories, all
of which draw air into the cabinet to minimize microbial
contamination back into the room.
What is the meaning of PPE? Name some examples. Ans:
Personal Protective Equipement
ex. laboratory coats, and gloves, eye, face protection
What component that is always present in experimental? Ans:
control or controls
What do microbiological experimentation tests determine?
Ans: The ability of an organism to use or produce some
chemical, or to determine the presence or absence of a specific
organism in a sample.
, 3
What does a positive result in the test indicate? Ans: The
microbe has the ability or is present in the sample.
What does a negative result in the test indicate? Ans: A lack of
that ability or absence in the sample
Tests should give a _________for specimens that are positive,
and a _________ for specimens that are negative. Ans: Positive;
negative
© 2026 Assignment
False positive is attributed to Ans: inadequate specificity
False negative is attributed to Ans: inadequate sensitivity
Guru01 - Stuvia
Expert
Inadequate specificity Ans: an inability to discriminate
between the chemical or organism in a question and similar
chemicals or organisms would yield false-positive results
which are similar chemicals or organism was being tested.
Inadequate sensitivity Ans: an inability to detect small
amounts of the chemical or organism in question would yield a
false negative result.
Write the formulas for Sensitivity Ans: True positives/True
positive + false negatives
Write the formulas for specificity Ans: True negatives/true
negatives + false positives
Biology is Ans: the study of living organism
Microbiology is Ans: an advanced biology course
Living microbes are known as Ans: cellular microbes or
microorganisms
