ONS/ONCC Chemotherapy Certification FULL STUDY
Exam , ACTUAL EXAMS & TESTS ( A GRADED 100%
VERIFIED)
Lesson 1: Foundations to Set the Stage -
Focusing on Cellular Structure and Function -
The Normal Cell Cycle - -The cell cycle refers to the ordered seres of processes of DNA replication and
mitosis, or cell division
-Cell nucleus regulates these processes by gathering and processing complexes molecular information
Interphase and Mitotic Phase - Cell division produces two identical cells through these two major
phases
During interphase: - Cell grows and DNA is replicated through the following three steps:
1: First growth phase (G1 or first gap)
2: Synthesis phase (S phase)
3:Mitotic Phse (M phase)
First Growth Phase (G1 or first gap) - -cells increase in size
-reproduce RNA
-"quality assurance" test that the cell will be ready to synthesis DNA
-Length of time is variable, can be from hours to days
Synthesis Phase (S phase) - -DNA replicates
-Results in the formation of identical pairs of DNA (chromatids)
-which are attached a t the centromere
-lasts 2-10 hours
1
,Mitotic Phase (M phase) - -Replicated chromosomes are aligned, separated, and move into 2 new,
identical daughter cells
-takes about 30-60 minutes
Major points of cell regulation are entry and exit from - -G1 checkpoint
-S Phase
-G2 checkpoint
-M phase
Restriction Point - -The transition from the resting phase into an actively dividing phase (G0-G1) is a
point where cellular transformation can occur
-During this time, cells pass through a transition phase known as a restriction point
-Extracellular growth factors trigger reentry into G1, and GF are required to send the cells past the
restriction point, or the point of no return
G0 Phase (resting phase) - -After mitosis, cells may enter back into the G1 phase or go into a resting
phase, known as G0
-Most cells in the human body reside in G0
-Exceptions to this are those that are (Resting in G0 phase) - -Exceptions to this are those that are
metabollically active, such as
-granulocytes
-and the epithelium of the GI tract
Cell Cycling Time - Amount of time from mitosis to mitosis
Cell cycle video and image -
http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/
animation__how_the_cell_cycle_works.html
Check points in the Cell Cycle: Keeping it All Under Control - -The cell cycle is carefully controlled
through a series of checkpoints
-Variation in duplication or distribution of chromosomes during cell division can alter the genetic
information passed on to daughter cells, leading to cellular dysfunction and disease, such as cancer
-These checkpoints monitor for DNA integrity and control progression through mitosis
2
, Progression through the cell cycle is controlled through two proteins: - 1. cyclines (D, E, A, B)
2. Cyclin-dependent kinases (CDKs)
-Cyclin-CDK complex allows the cell to progress through each phase of the cell cycle
Locations of proteins Cyclins (D, E, A, B) and CDKs - -(G0-G1) : Cyclin D and CDK 4/6
-Early S: Cyclin E and CDK 1/2
-Late S: Cyclin A and CDK 1/2
-G2: CDK 1/2 and cyclin A
-Before M: CDK 1 and Cyclin B
Inhibitory proteins - -prevent progression of the cycle when DNA damage is detected
-An example of an inhibitory protein is p53 (AKA TP53)
DNA Damage Checkpoints - -If DNA damage is present, cells are programmed to stop dividing or
undergo apoptosis (programmed cell death)
-The retinoblastoma protein (Rb), p53, and p21 are some of the most well-understood inhibitory
proteins (IP)
Inhibitory proteins p53 - -Levels of this IP regulate several important target genes
-Will increase when DNA damage is present
-Protects against inappropriate signal proliferation
-sometimes called the "suicide gene"
M Phase Checkpoints - When the cells prepare to divide, the chromosomes line up in the mitotic
spindle.
If the chromosomes are not properly aligned, division is not allowed to continue
Immunity -
Cells of the Immune System -
Pluripotent Stem Cell - -The cells of the immune system are created in the bome marrow from what is
know as a ___
3
Exam , ACTUAL EXAMS & TESTS ( A GRADED 100%
VERIFIED)
Lesson 1: Foundations to Set the Stage -
Focusing on Cellular Structure and Function -
The Normal Cell Cycle - -The cell cycle refers to the ordered seres of processes of DNA replication and
mitosis, or cell division
-Cell nucleus regulates these processes by gathering and processing complexes molecular information
Interphase and Mitotic Phase - Cell division produces two identical cells through these two major
phases
During interphase: - Cell grows and DNA is replicated through the following three steps:
1: First growth phase (G1 or first gap)
2: Synthesis phase (S phase)
3:Mitotic Phse (M phase)
First Growth Phase (G1 or first gap) - -cells increase in size
-reproduce RNA
-"quality assurance" test that the cell will be ready to synthesis DNA
-Length of time is variable, can be from hours to days
Synthesis Phase (S phase) - -DNA replicates
-Results in the formation of identical pairs of DNA (chromatids)
-which are attached a t the centromere
-lasts 2-10 hours
1
,Mitotic Phase (M phase) - -Replicated chromosomes are aligned, separated, and move into 2 new,
identical daughter cells
-takes about 30-60 minutes
Major points of cell regulation are entry and exit from - -G1 checkpoint
-S Phase
-G2 checkpoint
-M phase
Restriction Point - -The transition from the resting phase into an actively dividing phase (G0-G1) is a
point where cellular transformation can occur
-During this time, cells pass through a transition phase known as a restriction point
-Extracellular growth factors trigger reentry into G1, and GF are required to send the cells past the
restriction point, or the point of no return
G0 Phase (resting phase) - -After mitosis, cells may enter back into the G1 phase or go into a resting
phase, known as G0
-Most cells in the human body reside in G0
-Exceptions to this are those that are (Resting in G0 phase) - -Exceptions to this are those that are
metabollically active, such as
-granulocytes
-and the epithelium of the GI tract
Cell Cycling Time - Amount of time from mitosis to mitosis
Cell cycle video and image -
http://highered.mheducation.com/sites/0072495855/student_view0/chapter2/
animation__how_the_cell_cycle_works.html
Check points in the Cell Cycle: Keeping it All Under Control - -The cell cycle is carefully controlled
through a series of checkpoints
-Variation in duplication or distribution of chromosomes during cell division can alter the genetic
information passed on to daughter cells, leading to cellular dysfunction and disease, such as cancer
-These checkpoints monitor for DNA integrity and control progression through mitosis
2
, Progression through the cell cycle is controlled through two proteins: - 1. cyclines (D, E, A, B)
2. Cyclin-dependent kinases (CDKs)
-Cyclin-CDK complex allows the cell to progress through each phase of the cell cycle
Locations of proteins Cyclins (D, E, A, B) and CDKs - -(G0-G1) : Cyclin D and CDK 4/6
-Early S: Cyclin E and CDK 1/2
-Late S: Cyclin A and CDK 1/2
-G2: CDK 1/2 and cyclin A
-Before M: CDK 1 and Cyclin B
Inhibitory proteins - -prevent progression of the cycle when DNA damage is detected
-An example of an inhibitory protein is p53 (AKA TP53)
DNA Damage Checkpoints - -If DNA damage is present, cells are programmed to stop dividing or
undergo apoptosis (programmed cell death)
-The retinoblastoma protein (Rb), p53, and p21 are some of the most well-understood inhibitory
proteins (IP)
Inhibitory proteins p53 - -Levels of this IP regulate several important target genes
-Will increase when DNA damage is present
-Protects against inappropriate signal proliferation
-sometimes called the "suicide gene"
M Phase Checkpoints - When the cells prepare to divide, the chromosomes line up in the mitotic
spindle.
If the chromosomes are not properly aligned, division is not allowed to continue
Immunity -
Cells of the Immune System -
Pluripotent Stem Cell - -The cells of the immune system are created in the bome marrow from what is
know as a ___
3
