ACS Biochemistry Exam 5 Test Bank Verified Questions Answers 2025/ 2026

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ACS BIOCHEMISTRY EXAM | QUESTIONS AND ANSWERS | VERIFIED ANSWERS
GRADED A+ | LATEST EXAM




Step 1 of Glycolysis - CORRECT ANSWER - Glucose --> Glucose 6-
phosphate. Uses hexokinase enzyme.
ATP --> ADP




Step 2 of Glycolysis - CORRECT ANSWER - Glucose 6-phosphate <-->
Fructose 6-phosphate
Uses phosphohexose isomerase enzyme.




Step 3 of Glycolysis - CORRECT ANSWER - Fructose 6-phosphate -->
Fructose 1,6-bisphosphate
Uses PFK-1 (phosphofructokinase-1) enzyme. ATP --> ADP




First Committed Step of Glycolysis - CORRECT ANSWER - Step 3 of
Glycolysis. Fructose 6-Phosphate --> Fructose 1,6-bisphosphate. (PFK-1)




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Step 4 of Glycolysis - CORRECT ANSWER - Fructose 1,6-bisphosphate <-->
dihydroxyacetone + glyceraldehyde 3-phosphate.
Uses aldolase enzyme.




Step 5 of Glycolysis - CORRECT ANSWER - Dihydroxyacetonephosphate <-
-> glyceraldehyde 3-phosphate
Uses triose phosphate isomerase enzyme.




Step 6 of Glycolysis - CORRECT ANSWER - Glyceraldehyde 3-Phosphate +
Pi <-
-> 1,3-biphosphoglycerate.
Uses G3P dehydrogenase enzyme. NAD+ <--> NADH




First Energy Yielding Step of Glycolysis - CORRECT ANSWER - Step 6 of
Glycolysis.
G3P + Pi <--> 1,3-bisphosphoglycerate




Step 7 of Glycolysis - CORRECT ANSWER - 1,3-bisphosphoglycerate + ADP
<--> 3-phosphoglycerate + ATP




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Uses phosphoglycerate kinase enzyme.




First ATP Yielding Step of Glycolysis - CORRECT ANSWER - Step 7 of
Glycolysis.
1,3-bisphosphoglycerate <--> 3-phosphoglycerate




Step 8 of Glycolysis - CORRECT ANSWER - 3-phosphoglycerate <--> 2-
phosphoglycerate
Uses phosphoglycerate mutase enzyme.




Step 9 of Glycolysis - CORRECT ANSWER - 2-phosphoglycerate <-->
Phosphoenolpyruvate (PEP)
Uses enolase enzyme.
Dehydration reaction (loss of water).




Step 10 of Glycolysis - CORRECT ANSWER - PEP + ADP --> Pyruvate +
ATP Uses pyruvate kinase enzyme.




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ATP Consuming Steps of Glycolysis - CORRECT ANSWER - Step 1 and 3.
Glucose --> Glucose 6-phosphate
Fructose 6-phosphate --> Fructose 1,6-bisphosphate




ATP Producing Steps of Glycolysis - CORRECT ANSWER - Steps 7 and 10.
1,3-bisphosphoglycerate <--> 3-phosphoglycerate
PEP --> Pyruvate




NADH Producing Step of Glycolysis - CORRECT ANSWER - Step 6 G3P <--
> 1,3-bisphosphoglycerate




Total Energy Produced by Glycolysis - CORRECT ANSWER - 2NADH + 4
ATP




Lactic Acid Fermentation - CORRECT ANSWER - Pyruvate --> L-Lactate
NADH --> NAD+
Regenerates NAD+ for use in glycolysis




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