THEORY OF THE PRACTICALS
Theory of the practicals
Content
Lecture 1 03-02-‘21................................................................................................................................2
Introduction to C. elegans..................................................................................................................2
The life circle......................................................................................................................................2
The “childhood” years....................................................................................................................3
Life cycle timings of C. elegans.......................................................................................................3
Plates for worms.................................................................................................................................3
Food for worms..................................................................................................................................3
Picking C. elegans...............................................................................................................................4
Working with C. elegans.....................................................................................................................4
Communication:.............................................................................................................................4
Distinguish different development stages..........................................................................................4
Age synchronisation (bleaching).........................................................................................................5
Tips for excellent bleaching:...........................................................................................................5
Good literature places........................................................................................................................6
C. elegans worm strains......................................................................................................................6
Techniques to complete.....................................................................................................................6
RNAi........................................................................................................................................................7
Different methods to deliver RNAi to C. elegans................................................................................7
Plasmid L4440.....................................................................................................................................7
Lecture 2 – Techniques to complete in Tc10 24-02-‘21........................................................................10
Genome wide RNAi clone library......................................................................................................10
VIDAL RNAi library........................................................................................................................10
RNAi experiments.............................................................................................................................10
RNAi controls....................................................................................................................................11
RNAi GOI...........................................................................................................................................11
1
, THEORY OF THE PRACTICALS
Lecture 1 03-02-‘21
Introduction to C. elegans
Free-living, non-parasitic soil nematode (roundworm)
Small (~ 1 mm)
Feeds on microbes, primarily bacteria
Non-hazardous, non-infectios, non-pathogenic
Found throughout the world
Nickname “the worm”
The germ cells are produced at the distal gonad; they grow and develop till they reach the
spermatheca. After the fertilization they are called embryos and are nearby the vulva. Via the vulva
the eggs are released.
The life circle
2
Theory of the practicals
Content
Lecture 1 03-02-‘21................................................................................................................................2
Introduction to C. elegans..................................................................................................................2
The life circle......................................................................................................................................2
The “childhood” years....................................................................................................................3
Life cycle timings of C. elegans.......................................................................................................3
Plates for worms.................................................................................................................................3
Food for worms..................................................................................................................................3
Picking C. elegans...............................................................................................................................4
Working with C. elegans.....................................................................................................................4
Communication:.............................................................................................................................4
Distinguish different development stages..........................................................................................4
Age synchronisation (bleaching).........................................................................................................5
Tips for excellent bleaching:...........................................................................................................5
Good literature places........................................................................................................................6
C. elegans worm strains......................................................................................................................6
Techniques to complete.....................................................................................................................6
RNAi........................................................................................................................................................7
Different methods to deliver RNAi to C. elegans................................................................................7
Plasmid L4440.....................................................................................................................................7
Lecture 2 – Techniques to complete in Tc10 24-02-‘21........................................................................10
Genome wide RNAi clone library......................................................................................................10
VIDAL RNAi library........................................................................................................................10
RNAi experiments.............................................................................................................................10
RNAi controls....................................................................................................................................11
RNAi GOI...........................................................................................................................................11
1
, THEORY OF THE PRACTICALS
Lecture 1 03-02-‘21
Introduction to C. elegans
Free-living, non-parasitic soil nematode (roundworm)
Small (~ 1 mm)
Feeds on microbes, primarily bacteria
Non-hazardous, non-infectios, non-pathogenic
Found throughout the world
Nickname “the worm”
The germ cells are produced at the distal gonad; they grow and develop till they reach the
spermatheca. After the fertilization they are called embryos and are nearby the vulva. Via the vulva
the eggs are released.
The life circle
2
