HISTOPATHOLOGY COMPREHENSIVE
Exam part 1 UPDATED QUESTIONS AND
CORRECT ANSWERS
Teasing or Dissociation
Squashing or Crushing
Smearing
Frozen Section - CORRECT ANSWER Methods of Preparing Fresh Tissue Mounts
ISOTONIC SOLUTION - CORRECT ANSWER In teasing or Dissociation, the tissue
specimen is immersed in a watch glass containing what chemical?
Methylene blue (1-2 drops) - CORRECT ANSWER In squashing or Crushing, what is
added to the tissue slice on the glass slide directly?
Smearing - CORRECT ANSWER Method of choice for examining sediments (e.g.
fluids)
STREAKING - CORRECT ANSWER Materials is rapidly and gently applied in a
direct or zigzag line throughout the slide,
attempting to obtain a relatively uniform
distribution of secretion
SPREADING - CORRECT ANSWER Main advantage:
maintains cellular
interrelationships
oPortion of a material is gently spread into a
moderately thick film by teasing the
, strands apart with an applicator stick
SPREADING - CORRECT ANSWER Recommended for smear prep of
fresh
sputum, bronchial aspirates, and
thick
mucoid secretions
Liquid N2 (around -70 degrees Celsius)
Isopentane,
CO2
aerosol sprays - CORRECT ANSWER Used in frozen section
Commonly used for:
o Rapid pathologic dx during surgery
o Dx and research for enzyme histochemistry
o Dx and research demonstration of soluble substances such as lipids and carbohydrates
o Immunofluorescent and
immunohistochemical staining
o Some specialized silver stains (silver impregnation is the correct term), particularly in
neuropathology - CORRECT ANSWER Frozen sections are used for:
5-10 um - CORRECT ANSWER Tissue section size in Cryostat
-20 to -30 degrees Celsius - CORRECT ANSWER Tissue section in Cryostat is frozen
rapidly to about ___________
polyethylene glycol (PEG) and polyvinyl
Exam part 1 UPDATED QUESTIONS AND
CORRECT ANSWERS
Teasing or Dissociation
Squashing or Crushing
Smearing
Frozen Section - CORRECT ANSWER Methods of Preparing Fresh Tissue Mounts
ISOTONIC SOLUTION - CORRECT ANSWER In teasing or Dissociation, the tissue
specimen is immersed in a watch glass containing what chemical?
Methylene blue (1-2 drops) - CORRECT ANSWER In squashing or Crushing, what is
added to the tissue slice on the glass slide directly?
Smearing - CORRECT ANSWER Method of choice for examining sediments (e.g.
fluids)
STREAKING - CORRECT ANSWER Materials is rapidly and gently applied in a
direct or zigzag line throughout the slide,
attempting to obtain a relatively uniform
distribution of secretion
SPREADING - CORRECT ANSWER Main advantage:
maintains cellular
interrelationships
oPortion of a material is gently spread into a
moderately thick film by teasing the
, strands apart with an applicator stick
SPREADING - CORRECT ANSWER Recommended for smear prep of
fresh
sputum, bronchial aspirates, and
thick
mucoid secretions
Liquid N2 (around -70 degrees Celsius)
Isopentane,
CO2
aerosol sprays - CORRECT ANSWER Used in frozen section
Commonly used for:
o Rapid pathologic dx during surgery
o Dx and research for enzyme histochemistry
o Dx and research demonstration of soluble substances such as lipids and carbohydrates
o Immunofluorescent and
immunohistochemical staining
o Some specialized silver stains (silver impregnation is the correct term), particularly in
neuropathology - CORRECT ANSWER Frozen sections are used for:
5-10 um - CORRECT ANSWER Tissue section size in Cryostat
-20 to -30 degrees Celsius - CORRECT ANSWER Tissue section in Cryostat is frozen
rapidly to about ___________
polyethylene glycol (PEG) and polyvinyl
