an d answ er s w ith accu r ate
r ationales(PASSED)
Anton van Leeuwenhoek - answer Father of microbiology
Created 1st magnifying glass
Coined the term 'Animalcules'
Animalcules - answer First term used to describe microorganisms
What is the theory of Spontaneous Generation? - answer Life arises
spontaneously from non-living material (abiogenesis)
Who were the scientists involved in the theory of spontaneous generation? -
answer Francesco Redi (meat in jars experiment)
Louis Pasteur (Swan Neck Flask experiment)
John Tyndall (found heat resistant microbes)
Ferdinand Cohn (discovered endospores)
Robert Koch (discovered anthrax disease by spore forming bacteria)
What were the structures present in hay infusions? - answer Heat resistant
microbes called endospores
,Which experiment disproved TSG (theory of spontaneous generation)? - answer
Louis Pasteur swan neck flask experiment
What is bioremediation? - answer The use of microorganisms to clean pollution
How do viruses grow? - answer They cannot reproduce on their own. The must
use a living host cell
What does the resolving power of a microscope do? - answer Distinguish two
objects that are close together
What does immersion oil do? - answer Increases resolution and decreases light
refraction
Which microscope is used to observe a specimen at the atomic level? - answer
Atomic Force Microscope (1980)
What does positive staining do? - answer Contains positive charge that dyes the
cells
What does negative staining do? - answer Contains negative charge that dyes the
background
Which dyes contain a positive charge? - answer Basic dyes
, Which dyes contain a negative charge? - answer Acidic dyes
What is the advantage of the size of prokaryotes? - answer Their small size makes
it so they have a rapid growth rate
Steps used for a gram stain:
1: Crystal Violet 2: Iodine 3: Alcohol 4: Safranin
A: 1,3,4,2
B: 1,3,2,4
C: 2,3,1,4
D: 1,2,3,4 - answer D
Which step during the process of gram staining could cause for a gram+ stain to
appear to be a gram- stain? - answer Step 3, decolorization using alcohol
Why is it important to study microorganisms/bacteria? - answer We can not live
without them
What is the equation to find the total magnification from a microscope? - answer
Objective lens x Ocular lens
Diplococcus - answer pairs
Streptococcus - answer chains