ASM 275 FINAL PAPER 2026 COMPLETE
QUESTIONS ANSWERS
◉ uncontrolled diabetes mellitus. Answer: -acid base imbalance
-can be life threatening
◉ sodium fluoride specimen (determination of glucose). Answer: -
preserves glucose 24hrs at RT
-gray top tubes are the best for collecting glucose specimens because
they have a specific anticoagulant in the tube that prevents glucose
change
-if other enzyme assays are ordered, a red top will be used instead of
drawing a gray top (excessive work)
◉ wavelength. Answer: the distance between the crests of two
successive waves
◉ amplitude. Answer: the height of each wave
-little wave the is very long OR short wave that is very tall
◉ frequency. Answer: the number of waves which pass an
observation point in ONE second
-relates to speed and how fast the wave is moving
,◉ short. Answer: high energy and high frequency = (short/long)
wavelength
◉ long. Answer: low energy and low frequency = (short/long)
wavelength
◉ red, blue. Answer: (red/blue) is longer wavelength, (red/blue) is
shorter wavelength on visible spectrum
◉ reflected. Answer: the wave bounces back to the source
◉ absorbed. Answer: the wave is absorbed by the material
-light that comes in the solution and stays there
◉ transmitted. Answer: the wave passes through the material
-goes through the solution
◉ diffracted. Answer: the wave changes direction as they pass
through an opening or around a barrier
-bounces off at an angle
,◉ fluoresced. Answer: the wave is first absorbed then emitted at a
different (often longer and visible) wavelength
-absorbed at one wavelength and emitted at another
◉ transparent. Answer: -all light passes through
-think of a clear window
◉ translucent. Answer: -some light passes through
-like a frosted window (light comes through but can't really see
through it)
◉ opaque. Answer: -no light passes through
-like a closed door
◉ diffraction of light. Answer: can change in the direction a light
beam as it travels through a slit or by the edge of an opaque
solution/object
◉ diffraction of light through a slit. Answer: change in direction of a
light beam as it travels through a slit
-creates bending of light and makes different wavelengths visible
-create wavelengths through diffraction grating to measure the
specific analyte
, -send white light through a slit
-slit creates bending of the light
-bending of light will create multiple visible wavelengths (similar to
a prism)
◉ diffraction of light by the edge of an opaque solution/object.
Answer: change in direction of a light beam as it travels by the edge
of an opaque solution/object
-putting light against an object and it bends
-red bends the least (due to longer wavelength)
-blue bends the most (due to shorter wavelength)
◉ Bouger's. Answer: percent transmittance and absorbance is
measured by _____ law
◉ Bouger's Law equation. Answer: light transmitted/original
(incident) light = I/Io = 1 = 100%T
-Io: light source (shines the light)
-I: transmitted light (light that comes out on the other side)
-100%T: all of the light that is passing through the solution
◉ Bouger's Law steps. Answer: -light hits monochromator
-isolates the wavelength
QUESTIONS ANSWERS
◉ uncontrolled diabetes mellitus. Answer: -acid base imbalance
-can be life threatening
◉ sodium fluoride specimen (determination of glucose). Answer: -
preserves glucose 24hrs at RT
-gray top tubes are the best for collecting glucose specimens because
they have a specific anticoagulant in the tube that prevents glucose
change
-if other enzyme assays are ordered, a red top will be used instead of
drawing a gray top (excessive work)
◉ wavelength. Answer: the distance between the crests of two
successive waves
◉ amplitude. Answer: the height of each wave
-little wave the is very long OR short wave that is very tall
◉ frequency. Answer: the number of waves which pass an
observation point in ONE second
-relates to speed and how fast the wave is moving
,◉ short. Answer: high energy and high frequency = (short/long)
wavelength
◉ long. Answer: low energy and low frequency = (short/long)
wavelength
◉ red, blue. Answer: (red/blue) is longer wavelength, (red/blue) is
shorter wavelength on visible spectrum
◉ reflected. Answer: the wave bounces back to the source
◉ absorbed. Answer: the wave is absorbed by the material
-light that comes in the solution and stays there
◉ transmitted. Answer: the wave passes through the material
-goes through the solution
◉ diffracted. Answer: the wave changes direction as they pass
through an opening or around a barrier
-bounces off at an angle
,◉ fluoresced. Answer: the wave is first absorbed then emitted at a
different (often longer and visible) wavelength
-absorbed at one wavelength and emitted at another
◉ transparent. Answer: -all light passes through
-think of a clear window
◉ translucent. Answer: -some light passes through
-like a frosted window (light comes through but can't really see
through it)
◉ opaque. Answer: -no light passes through
-like a closed door
◉ diffraction of light. Answer: can change in the direction a light
beam as it travels through a slit or by the edge of an opaque
solution/object
◉ diffraction of light through a slit. Answer: change in direction of a
light beam as it travels through a slit
-creates bending of light and makes different wavelengths visible
-create wavelengths through diffraction grating to measure the
specific analyte
, -send white light through a slit
-slit creates bending of the light
-bending of light will create multiple visible wavelengths (similar to
a prism)
◉ diffraction of light by the edge of an opaque solution/object.
Answer: change in direction of a light beam as it travels by the edge
of an opaque solution/object
-putting light against an object and it bends
-red bends the least (due to longer wavelength)
-blue bends the most (due to shorter wavelength)
◉ Bouger's. Answer: percent transmittance and absorbance is
measured by _____ law
◉ Bouger's Law equation. Answer: light transmitted/original
(incident) light = I/Io = 1 = 100%T
-Io: light source (shines the light)
-I: transmitted light (light that comes out on the other side)
-100%T: all of the light that is passing through the solution
◉ Bouger's Law steps. Answer: -light hits monochromator
-isolates the wavelength
