Test Bank - Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications, 3rd Edition (Buckingham, 2019), Chapter 1-15 | All Chapters

TEST BANK
Molecular Diagnostics: Fundamentals, Methods, and Clinical
Applications

Lela Buckingham
3rd Edition

,Table of Contents

Chapter 01 Nucleic Acids and Proteins 1
Chapter 02 Gene Expression and Epigenetics 14
Chapter 03 Nucleic Acid Extraction Methods 19
Chapter 04 Resolution and Detection of Nucleic Acids 25
Chapter 05 Analysis and Characterization of Nucleic Acids and Proteins 31
Chapter 06 Nucleic Acid Amplification 37
Chapter 07 Chromosomal Structure and Chromosomal Mutations 43
Chapter 08 Gene Mutations 49
Chapter 09 DNA Sequencing 55
Chapter 10 DNA Polymorphisms and Human Identification 61
Chapter 11 Detection and Identification of Microorganisms 67
Chapter 12 Molecular Detection of Inherited Diseases 73
Chapter 13 Molecular Oncology 79
Chapter 14 DNA-Based Tissue Typing 85
Chapter 15 Quality Assurance and Quality Control in the Molecular Laboratory 91

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Test Bank - Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications, 3rd Edition (Buckingham, 2019)

Chapter 1: Nucleic Acids and Proteins


MULTIPLE CHOICE

1. DNA contains deoxyribonucleotide triphosphates joined together by what type of covalent
bond?
A. Aldehyde
B. Ester
C. Phosphodiester
D. Peptide
ANS: C OBJ: 1-2

2. Nucleotides in DNA consist of which of the following?
A. Nitrogen base, deoxyribose, and phosphate
B. Nitrogen base, ribose, and sulfur
C. Carbon base, ribose, and phosphate
D. Carbon base, glucose, and carboxyl
ANS: A OBJ: 1-1

3. Which of the five ribose carbons of the deoxyribonucleotide are involved in the formation of
the DNA chain?
A. 1' and 5'
B. 2' and 4'
C. 3' and 4'
D. 3' and 5'
ANS: D OBJ: 1-23

4. In a DNA molecule, one end has a free hydroxyl group, and one end has a free
A. phosphate group.
B. hydroxyl group.
C. amino group.
D. carboxyl group.
ANS: A OBJ: 1-1

5. What type of bonds spontaneously form between two complementary strands of DNA?
A. Hydrogen bonds
B. Covalent bonds
C. Phosphodiester bonds
D. Polar covalent bonds
ANS: C OBJ: 1-2

6. In a double-stranded DNA molecule, base pairing between strands occurs between
A. a purine and a purine.
B. a pyrimidine and a pyrimidine.
C. a purine and a pyrimidine.



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Test Bank - Molecular Diagnostics: Fundamentals, Methods, and Clinical Applications, 3rd Edition (Buckingham, 2019)

D. all types of nucleotide bases.
ANS: C OBJ: 1-1

7. Which of the following is a purine?
A. Thymine
B. Cytosine
C. Adenine
D. Alanine
ANS: C OBJ: 1-1

8. Which DNA sequence is complementary to 5'GATTCTCAAAGGACT3'?
A. 5'GATTCTCAAAGGACT3'
B. 3'GATTCTCAAAGGACT5'
C. 3'CTAAGAGTTTCCTGA5'
D. 5'CTAAGAGTTTCCTGA3'
ANS: C OBJ: 1-7

9. The term used to describe the arrangement of the individual strands in the double-stranded
DNA molecule is
A. parallel.
B. antiparallel.
C. tangential.
D. divergent.
ANS: B OBJ: 1-7

10. The process of separating the two DNA strands into two single strands is called
A. denaturation.
B. bidirectional.
C. depolymerization.
D. synthesis.
ANS: A OBJ: 1-2

11. DNA replication is
A. conservative.
B. semiconservative.
C. nonconservative.
D. dispersive.
ANS: B OBJ: 1-3

12. DNA replication requires the presence of
A. DNA template.
B. ribosomes.
C. amino acids.
D. messenger RNA.
ANS: A OBJ: 1-3



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