MICRO FINAL EXAM PORTAGE
LEARNING
A dilution gradient is formed when carrying out what generalize
plating strategy? - ANSWERS-Phase-Dilution streak
In what phase of a dilution streak would you expect to find the highest
concentration of bacteria, P2 or P4? - ANSWERS-P2
True or False. When performing a dilution streak a new (or sterilized)
loop is not required for each phase as long as the bacterial culture is
pure. - ANSWERS-False
The number of phases (3 vs. 4) and/or the number of times a loop
passes through a previous phase (once vs. multiple times) is
acceptable provided what happens? - ANSWERS-The resulting
gradient has in it the growth of individual colonies.
True or False. To restrict the growth of a pathogenic microbe a
researcher might decrease an incubator from 37°C to 25°C. -
ANSWERS-True
When given an unknown bacterial sample the first step is to expand
the current bacterial population. Which form of media best suites this
need? Why? - ANSWERS-LB media because all of the other forms
, are selective media meaning they may potentially inhibit the growth
of the unknown sample.
True or False. Only directly ingesting the pre-formed clostridium
toxin (neither the bacteria nor its spores) will cause disease in adults. -
ANSWERS-True
True or False. There are currently no cures for tetanus. - ANSWERS-
True
The alpha-toxin perfringolysin is associated with which medical
condition caused by Gram-positive anaerobic bacteria?
Gas gangrene
Tetanus
Leprosy
Botulism - ANSWERS-Gas gangrene
True or False. Bubonic plague is often characterized by painfully
swollen lymph nodes. - ANSWERS-True
List the following disease: - ANSWERS-Anthrax
True or False. Chlyamida is an obligate parasite and as such can be
grown on agar plates alone. - ANSWERS-False
LEARNING
A dilution gradient is formed when carrying out what generalize
plating strategy? - ANSWERS-Phase-Dilution streak
In what phase of a dilution streak would you expect to find the highest
concentration of bacteria, P2 or P4? - ANSWERS-P2
True or False. When performing a dilution streak a new (or sterilized)
loop is not required for each phase as long as the bacterial culture is
pure. - ANSWERS-False
The number of phases (3 vs. 4) and/or the number of times a loop
passes through a previous phase (once vs. multiple times) is
acceptable provided what happens? - ANSWERS-The resulting
gradient has in it the growth of individual colonies.
True or False. To restrict the growth of a pathogenic microbe a
researcher might decrease an incubator from 37°C to 25°C. -
ANSWERS-True
When given an unknown bacterial sample the first step is to expand
the current bacterial population. Which form of media best suites this
need? Why? - ANSWERS-LB media because all of the other forms
, are selective media meaning they may potentially inhibit the growth
of the unknown sample.
True or False. Only directly ingesting the pre-formed clostridium
toxin (neither the bacteria nor its spores) will cause disease in adults. -
ANSWERS-True
True or False. There are currently no cures for tetanus. - ANSWERS-
True
The alpha-toxin perfringolysin is associated with which medical
condition caused by Gram-positive anaerobic bacteria?
Gas gangrene
Tetanus
Leprosy
Botulism - ANSWERS-Gas gangrene
True or False. Bubonic plague is often characterized by painfully
swollen lymph nodes. - ANSWERS-True
List the following disease: - ANSWERS-Anthrax
True or False. Chlyamida is an obligate parasite and as such can be
grown on agar plates alone. - ANSWERS-False
