Molecular Cell Biology.pdf

, 1.
Which of the following enzymes will produce a blunt end ? (Cut site is indicated by the *
in the recognition sequence.)
A) TaqI (T*CGA)
B) EagI (C*GGCCG)
C) EcoRV (GAT*ATC)
D) NsiI (ATGCA*T)
E) BamHI (G*GATCC) - CORRECT ANSWERS-C

2.
The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA
sequencing is to
A) Denature DNA into single strands
B) Serve as primers
C) Be a fluorescent tag
D) Incorporate into newly replicated DNA strands and terminate their synthesis
E) None of the choices are correct - CORRECT ANSWERS-D

3.
The use of an antibiotic-resistance gene on a plasmid used in genetic engineering
makes
A) Replica plating possible.
B) Direct selection possible.
C) The recombinant cell dangerous.
D) The recombinant cell unable to survive.
E) All of the above. - CORRECT ANSWERS-B

4.
The following steps must be performed to make a bacterium produce human protein X:
1-Translation; 2-Restriction enzyme; 3-Prokaryotic transcription; 4-DNA ligase; 5-
Transformation; 6-Eukaryotic transcription; 7-Reverse transcription. Put the steps in the
correct sequence.
A) 5, 2, 3, 4, 7, 6, 1
B) 1, 2, 3, 5, 4, 7, 6
C) 6, 7, 2, 3, 4, 5, 1
D) 6, 7, 2, 4, 5, 3, 1
E) 6, 2, 1, 3, 4, 5, 7 - CORRECT ANSWERS-D

5.
Southern blotting is used to detect a specific
A) DNA.
B) RNA.
C) Protein.
D) Carbohydrate.