SC 165/166 Microbiology
MICROSCOPY
, Introduction to Microscopy
• Microscopes
– Compound light microscope
– SEM (scanning electron microscope
– TEM (transmission electron microscope
– Atomic force microscope
– Phase-contrast microscope
• Microscope terms
– Magnification
– Objective lenses (4x scanning, 10x low power, 40x high power, 100x oil
immersion)
– Contrast (important for single cell viewing)
– Wet mount versus dry mount
– Simple (using a single dye) versus differential (multi-step process to
distinguish organisms from one another) staining (Exs: gram, acid-fast
staining)
MICROSCOPY
, Introduction to Microscopy
• Microscopes
– Compound light microscope
– SEM (scanning electron microscope
– TEM (transmission electron microscope
– Atomic force microscope
– Phase-contrast microscope
• Microscope terms
– Magnification
– Objective lenses (4x scanning, 10x low power, 40x high power, 100x oil
immersion)
– Contrast (important for single cell viewing)
– Wet mount versus dry mount
– Simple (using a single dye) versus differential (multi-step process to
distinguish organisms from one another) staining (Exs: gram, acid-fast
staining)
