MCB 2050 Midterm Exam Questions
with All Correct Answers
When double stranded DNA splits into two single stranded molecules this is called... -
Answer-Denaturation
The term molecular hybridization refers to: - Answer-annealing of single stranded DNA
or RNA molecules to those with complimentary sequences
What would be expected to decreases the melting temperature of double stranded
DNA? - Answer-low G-C content and High A-T content
Which cells of the immune system are responsible for producing secreted antibody? -
Answer-not stem cells
You wish to study the protein-protein interactions of a specific protein. Which of the
following techniques is best suited for this task? - Answer-Co-Immunoprecipitation
You are studying the genes involved in embryo development. Which of the following
techniques could be used to visualize the locations with high expression of a particular
gene of interest in a whole mounted embryo? - Answer-In situ?
Put the following steps of a Southern Blot in the correct order.
1. The membrane is incubated with radio-labeled probes
2. Sample DNA is broken into fragments and run on an electrophoresis gel.
3. The membrane is visualized via X-ray and an autoradiogram is generated.
4. Sample DNA is transferred from the electrophoresis gel to a solid membrane. -
Answer-2, 4, 1, 3
What type of bonds are broken when double stranded DNA is denatured? - Answer-not
phosphodiester
What affects the melting temperature of double stranded DNA? - Answer-molecule
length
A southern Blot is used to analyze _______, while a northern blot is used to
analyze_______ - Answer-DNA, RNA
, G-C base pairs contain ____ hydrogen bonds, while A-T pairs contain ____ - Answer-3,
2
Which technique could be used to produce a human karyotype? - Answer-Fluorescence
in situ hybridization
The spilceosome consists of:
1. snRPS
2. snRNAs
3. U1
4. U4
5. all of the above - Answer-all of the above
The branch point A does not participate in base pairing with the ________ to allow for
the first ________ reaction in RNA splicing via the ________ group of the branch point
A.
A. U1 snRNA; polymerization; 3' OH
B. 3' splice site; debranching; 5' PO4
C. 3' splice site; transesterification; 3' OH
D. U2 snRNA; transesterification; 2' OH
E. U2 snRNA; debranching; 2' OH - Answer-D
Place the following events of RNA splicing in the correct sequence:
I. Formation of a 2' 5' phosphodiester bond between the branch point and 5' end of the
intron
II. The first transesterification reaction, catalyzed by U2 and U6
III. Recruitment of U3, U4, and U5 to the preRNA
IV. Binding of SF1 to the branch point A
V. Joining of the two exons via a 3' 5' phosphodiester bond
A.IV, III, II, I, V
B.III, IV, II, I, V
C.IV, III, I, II, V
D.III, IV, II, V, I
E.II, IV, III, I, V - Answer-A
What is splicing - Answer-joining of two exons and removal of an intron
What are the master regulators called that control the events of the cell cycle? -
Answer-Cyclin Dependent Kinases (CDK)
What is the regulatory subunit of CDKs called? - Answer-cyclins
with All Correct Answers
When double stranded DNA splits into two single stranded molecules this is called... -
Answer-Denaturation
The term molecular hybridization refers to: - Answer-annealing of single stranded DNA
or RNA molecules to those with complimentary sequences
What would be expected to decreases the melting temperature of double stranded
DNA? - Answer-low G-C content and High A-T content
Which cells of the immune system are responsible for producing secreted antibody? -
Answer-not stem cells
You wish to study the protein-protein interactions of a specific protein. Which of the
following techniques is best suited for this task? - Answer-Co-Immunoprecipitation
You are studying the genes involved in embryo development. Which of the following
techniques could be used to visualize the locations with high expression of a particular
gene of interest in a whole mounted embryo? - Answer-In situ?
Put the following steps of a Southern Blot in the correct order.
1. The membrane is incubated with radio-labeled probes
2. Sample DNA is broken into fragments and run on an electrophoresis gel.
3. The membrane is visualized via X-ray and an autoradiogram is generated.
4. Sample DNA is transferred from the electrophoresis gel to a solid membrane. -
Answer-2, 4, 1, 3
What type of bonds are broken when double stranded DNA is denatured? - Answer-not
phosphodiester
What affects the melting temperature of double stranded DNA? - Answer-molecule
length
A southern Blot is used to analyze _______, while a northern blot is used to
analyze_______ - Answer-DNA, RNA
, G-C base pairs contain ____ hydrogen bonds, while A-T pairs contain ____ - Answer-3,
2
Which technique could be used to produce a human karyotype? - Answer-Fluorescence
in situ hybridization
The spilceosome consists of:
1. snRPS
2. snRNAs
3. U1
4. U4
5. all of the above - Answer-all of the above
The branch point A does not participate in base pairing with the ________ to allow for
the first ________ reaction in RNA splicing via the ________ group of the branch point
A.
A. U1 snRNA; polymerization; 3' OH
B. 3' splice site; debranching; 5' PO4
C. 3' splice site; transesterification; 3' OH
D. U2 snRNA; transesterification; 2' OH
E. U2 snRNA; debranching; 2' OH - Answer-D
Place the following events of RNA splicing in the correct sequence:
I. Formation of a 2' 5' phosphodiester bond between the branch point and 5' end of the
intron
II. The first transesterification reaction, catalyzed by U2 and U6
III. Recruitment of U3, U4, and U5 to the preRNA
IV. Binding of SF1 to the branch point A
V. Joining of the two exons via a 3' 5' phosphodiester bond
A.IV, III, II, I, V
B.III, IV, II, I, V
C.IV, III, I, II, V
D.III, IV, II, V, I
E.II, IV, III, I, V - Answer-A
What is splicing - Answer-joining of two exons and removal of an intron
What are the master regulators called that control the events of the cell cycle? -
Answer-Cyclin Dependent Kinases (CDK)
What is the regulatory subunit of CDKs called? - Answer-cyclins
