UNE biochem Unit 1 Test Questions And Answers. Allopurinol is an inhibitor of Xanthine Oxidase. Addition of this compound will result in a decrease in what compound? Uric Acid DNA replication occurs during which phase of the cell cycle? S phase The affin

UNE Biochem Midterm Questions With Actual Answers. Changes in the physiological variables listed below can alter the affinity of hemoglobin for oxygen. Which of the following will lower the affinity of hemoglobin for oxygen? increase 2,3 biphosphoglycerate (BPG) Allosteric activators of hemoglobin will increase substrate binding through which of the following mechanisms? Binding the protein, and keeping it in the "R" conformation Which of the following is the best description of primary protein structure? A newly synthesized strand of amino acids Movement of ammonia from an amino acid to an α-keto acid involves a family of enzymes best categorized as which of the following? transferases A 19-year-old boy is diagnosed with Creutzfeldt-Jakob Disease which is caused by the introduction of an amyloid fold in the disease-causing protein. The introduction of this fold causes the protein to transition from a primarily α-helical structure to an aggregate of mostly β-sheets. This change in protein structure (leading to disease) is best attributed to changes in which of the following? folding of the secondary structures Which of the following best describes tertiary structure? Interactions between α-helices and β sheets to form a domain`` An enzyme has a mutation within the substrate binding site that reduces the binding of the coenzyme needed for covalent catalysis. Which of the following is likely to result as a consequence of this mutation? The enzyme will not be able to form the transition state complex Domains are common structural elements that retain a particular function within the protein. Which of the following is an example of a protein domain? An arrangement of β-strands connected by α-helices (βαβαβ) Under low energy conditions, AMP will bind to phosphofructokinase I and glycogen phosphorylase at site unique to the active site. This enhances the activity of these enzymes. This is an example of which of the follow types of enzyme regulation? Allosteric activation If a mutation is made within the active site of an enzyme resulting in a decrease in Km, which of the following will be true with respect to the enzyme kinetics? The enzyme will require a lower substrate concentration to reach ½Vmax` A 28-year-old male is recently diagnosed with HIV. As part of his treatment he is prescribed Nevirapine which interferes with retroviral reverse transcriptase enzyme. Using the graph below, which of the following best describes the impact of this drug on its target? it reduces the catalytic activity of the enzyme In the image below the blue line indicates enzyme kinetics with no inhibitor present. Based on this information which of the following is true? The green line shows enzyme kinetics with the addition of a noncompetitive inhibitor