1
BIOCHEM EXAM 2 QUESTIONS, Midterm
Questions with Correct Answers | Updated
(100% Correct Answers)
Which of the following enzymes is part of both the TCA cycle and
ETC? Answer: Succinate dehydrogenase
In the case of deficiency in phenylalanine hydroxlase which of the
following amino acids will become essential Answer: Tyrosine
HFI is a rare recessive disorder. Which of the following is not a
consequence of this enzyme deficiency? Answer: They will have low
blood sugar (hypoglycemia), especially after eating fruits, fructose,
sucrose, jaundice, excessive sleepiness, convulsions, irritability
When the pancreatic beta cells sense an increase in glucose, the
release of insulin is facilitated through which of the following
mechanisms Answer: Increase in intracellular calcium
During aerobic exercise, intracellular calcium increases and which of
the following will occur? Answer: Isocitrate dehydrogenase and
alpha-keto glutarate dehydrogenase are allosterically activated by
increase in calcium
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,2
Within the red blood cell, the lack of which of the following
compounds can result in exercessive hemolysis and jaundice?
Answer: NADPH
After fasting for 6 hours which of the following would be consistent
with hormone levels of this individual? Answer: Insulin (-), Glucagon
(+), Cortisol (+), Epinephrine (+)
There is a gain of function mutation in the insulin receptor substrate
that makes it permanently bound to the insulin receptor substrate
that makes it permanently bound to the insulin receptor. Which of
the following may occur as a result of this mutation Answer:
Increase in protein kinase B activity
Which of the following activators is correctly paired with the enzyme
that activates it? Answer: Fructose-2,6-bisphosphate :
phosphofructokinase 1
NOT:
Glucose-6-phosphate : hexokinase
ATP : alpha-ketogluterate dehydrogenase
ATP : pyruvate kinase
Citrate : citrate synthase
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,3
Under fed conditions, glucose taken up by the liver is primarily used
for which of the following processes? Answer: substrate for liver
glycogen synthesis
Which of the following process is considered to be catabolic?
Answer: Glycogenolysis
In an individual with a deficiency in PDC, which of the following
reactions would be directly impaired? Answer: Pyruvate -> Acetyl
CoA
Monocytes are unique cells that secrete the cytokine, interleukin-1.
Once released, this cytokine will bind a receptor on the outside of
the monocytes and regulate its response. In this situation
interleukin-1 is best described as which of the following? Answer:
an autocrine signal
NADH generated in the cytosol must be transported across the
mitochondrial membrane in order to be oxidized by the electron
transport chain. Which of the following shuttles or transporters can
be used to facilitate the movement of cytosolic NADH into the
mitochondria? Answer: Malate-aspartate shuttle
Based on the following schematic what will the charge be on
guanine pH of 10? Answer: -2
© 2025 All rights reserved
, 4
Which of the following is an example of mechanical work? Answer:
Cleavage of high energy phosphate bond to facilitate muscle
contraction
Which of the following TCA cycle intermediates is directly used to
synthesize heme? Answer: Succinyl CoA
Which of the following hormones will facilitate activation of protein
kinase A? Answer: Activation of G protein, activation of adenylate
cyclase, production of cAMP
Glucagon
Epinephrine
The ribonucleic reductase is regulated at both an activity site and a
substrate specificity site. Which of the following compounds must
always be bound to the activity site for an enzyme to be active?
Answer: ATP
An individual consumes a breakfast consisting of cereal, eggs,
sausage, pancakes and maple syrup. After this meal, which of the
following statements best summarizes his metabolic state? Answer:
Fructose 2,6 bisphosphate will be elevated
Which of the following digestive enzymes is primarily responsible
for digesting large polysaccharides chains into smaller
oligosaccharides? Answer: Alpha amylase
© 2025 All rights reserved
BIOCHEM EXAM 2 QUESTIONS, Midterm
Questions with Correct Answers | Updated
(100% Correct Answers)
Which of the following enzymes is part of both the TCA cycle and
ETC? Answer: Succinate dehydrogenase
In the case of deficiency in phenylalanine hydroxlase which of the
following amino acids will become essential Answer: Tyrosine
HFI is a rare recessive disorder. Which of the following is not a
consequence of this enzyme deficiency? Answer: They will have low
blood sugar (hypoglycemia), especially after eating fruits, fructose,
sucrose, jaundice, excessive sleepiness, convulsions, irritability
When the pancreatic beta cells sense an increase in glucose, the
release of insulin is facilitated through which of the following
mechanisms Answer: Increase in intracellular calcium
During aerobic exercise, intracellular calcium increases and which of
the following will occur? Answer: Isocitrate dehydrogenase and
alpha-keto glutarate dehydrogenase are allosterically activated by
increase in calcium
© 2025 All rights reserved
,2
Within the red blood cell, the lack of which of the following
compounds can result in exercessive hemolysis and jaundice?
Answer: NADPH
After fasting for 6 hours which of the following would be consistent
with hormone levels of this individual? Answer: Insulin (-), Glucagon
(+), Cortisol (+), Epinephrine (+)
There is a gain of function mutation in the insulin receptor substrate
that makes it permanently bound to the insulin receptor substrate
that makes it permanently bound to the insulin receptor. Which of
the following may occur as a result of this mutation Answer:
Increase in protein kinase B activity
Which of the following activators is correctly paired with the enzyme
that activates it? Answer: Fructose-2,6-bisphosphate :
phosphofructokinase 1
NOT:
Glucose-6-phosphate : hexokinase
ATP : alpha-ketogluterate dehydrogenase
ATP : pyruvate kinase
Citrate : citrate synthase
© 2025 All rights reserved
,3
Under fed conditions, glucose taken up by the liver is primarily used
for which of the following processes? Answer: substrate for liver
glycogen synthesis
Which of the following process is considered to be catabolic?
Answer: Glycogenolysis
In an individual with a deficiency in PDC, which of the following
reactions would be directly impaired? Answer: Pyruvate -> Acetyl
CoA
Monocytes are unique cells that secrete the cytokine, interleukin-1.
Once released, this cytokine will bind a receptor on the outside of
the monocytes and regulate its response. In this situation
interleukin-1 is best described as which of the following? Answer:
an autocrine signal
NADH generated in the cytosol must be transported across the
mitochondrial membrane in order to be oxidized by the electron
transport chain. Which of the following shuttles or transporters can
be used to facilitate the movement of cytosolic NADH into the
mitochondria? Answer: Malate-aspartate shuttle
Based on the following schematic what will the charge be on
guanine pH of 10? Answer: -2
© 2025 All rights reserved
, 4
Which of the following is an example of mechanical work? Answer:
Cleavage of high energy phosphate bond to facilitate muscle
contraction
Which of the following TCA cycle intermediates is directly used to
synthesize heme? Answer: Succinyl CoA
Which of the following hormones will facilitate activation of protein
kinase A? Answer: Activation of G protein, activation of adenylate
cyclase, production of cAMP
Glucagon
Epinephrine
The ribonucleic reductase is regulated at both an activity site and a
substrate specificity site. Which of the following compounds must
always be bound to the activity site for an enzyme to be active?
Answer: ATP
An individual consumes a breakfast consisting of cereal, eggs,
sausage, pancakes and maple syrup. After this meal, which of the
following statements best summarizes his metabolic state? Answer:
Fructose 2,6 bisphosphate will be elevated
Which of the following digestive enzymes is primarily responsible
for digesting large polysaccharides chains into smaller
oligosaccharides? Answer: Alpha amylase
© 2025 All rights reserved
