Crispr cas9 Study guides, Study notes & Summaries

CRISPR Cas-9 Semester 1 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM De...

CMMB 403 final exam what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - Answer ️️ -- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - Answer ️️ -endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - Answer ️️ -1. non-homologous end joining (NHEJ) - nat...

CRISPR-Cas9 Final Practice Exam Questions 2024.What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of re...

Molecular Diagnostics Chapter 5 Exam What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments How do you perform restriction mapping? ...

MMSC490 Practice Exam 1|123 Questions with Verified Answers cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. - CORRECT ANSWER a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a smal...

....Genetic modification can be seen as a consequential process. With some educational background of what it is and how it benefits the population, there could be a future.... This document and unit have been graded at distinction and talk about the Basic DNA techniques and genetic engineering technology. In my merit I have given examples of GE used in medicine and industry these are: Human insulin, spider silk, genetically modified crops, xenotransplantation, and stems cells. In distinction the...

Nucleotide - ANS deoxyribose, a 5C sugar with a hydroxyl group on the 3' carbon and a phosphate group on the 5' carbon, and a nitrogen base attached to the 1' carbon Amino acid - ANS coded 5' to 3' (N terminus to C terminus) Central dogma - ANS describes the flow of information from DNA (genotype) to RNA (portable) to protein (phenotype) via transcription and translation Codon - ANS A specific sequence of three adjacent bases on a strand of DNA o...

CRISPR-Cas9 Complete Study Guide Practice Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kin...

CRISPR Cas Written Exam Questions With 100% Correct Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Descr...

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