Gel electrophoresis Study guides, Study notes & Summaries

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far...

Prescott's Microbiology, 11e (Willey) Chapter 1 The Evolution of Microorganisms and Microbiology 1) Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. Answer: FALSE Topic: Taxonomy of Microorganisms Bloom's/Accessibility: 2. Understand / Keyboard Navigation ASM Topic: Module 01 Evolution ASM Objective: 01.05 The evolutionary relatedness of organisms is best reflected in phylogenetic trees. Learning Outcome: 01.02a Propose a timeline of the ori...

MB (ASCP) 2020(updated) correctly answeredConsider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Tested PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 12.42...

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far...

MB ASCP Test Molecular Techniques (20-30%) Questions & Answers 2024 What are the 3 types of gel electrophoresis - ANSWER-Agarose Polyacrylamide Pulse Field What is gel electrophoresis - ANSWER-the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis - ANSWER-due to DNA being negatively charged, it will migrate towards the positive end What determines how far...

Catalyst - ANSWER-a substance that increases the rate of a chemical reaction without being consumed Enzyme - ANSWER-a molecule that increases the rate of a chemical reaction without being consumed (ex: protein) Activation energy - ANSWER-amount of energy required to convert a substrate into a product Active site - ANSWER-location on an enzyme that physically interacts with the substrate How do enzymes catalyze reactions? - ANSWER-1. substrate attaches to active site 2. activation en...

Quiz 2 Biochemistry Lab Chem 378 Name:____________________ 1. Below are restriction enzyme maps of two plasmids. Draw or described in numbers the expected bands of digestion in 1% agarose gel electrophoresis, relative to the 2 bands identified for you as control. 2. Explain the different steps that are involved in a PCR experiment (include all the reagents used in the PCR mixture). 5.5 kb BamHI 3730 PstI 2650 BamHI 3050 PstI 1720 PstI BamH1 PstI BamHI pCAG-EYFP 4000 bp 3000 ...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2023/2024 COMPLETE SOLUTIONS LATEST 2023/2024 Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Use d in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solu...

AANP Board Questions With Correct Answers 2023 INR values below ____ increase stroke risk sixfold. - CORRECT ANSWER-2 What allergy contradicts the use of thiazide diuretics? - CORRECT ANSWER-Sulfa Why should ACE-Is be avoided with renal stenosis? - CORRECT ANSWER-Leads to ARF Adverse effects of aldosterone antagonists (Aldactone) - CORRECT ANSWERGalactorrhea and hyperkalemia What are beta blocker indications? - CORRECT ANSWER-HTN, post-MI< angina, arrhythmias, migraine prophylaxis...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...